FastQCFastQC Report
Fri 24 May 2024
EGAF00002529164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86982563
Total Bases12.3 Gbp
Sequences flagged as poor quality0
Sequence length1-144
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAAGACTTGAGATTGAGGACTGGCCAACAGGGTCAAAATGCAAGGCCT14036691.6137360771951499No Hit
CCATATCATTCTTGCCTGTGTACATACATCTCTTCTCCACTGCTCAGAAC13422071.543075938104974No Hit
AGACATTTCAAAATGACCCTGTAGGCAAGGCCCAGACATAAAAGTTACAT11698581.344933926584803No Hit
CTCTGGCTTGATCCTTGTATATGAGCCCTAGCATCCAGTCCAAGTATTTC11480241.319832343868736No Hit
GATTATGGTCGCTGAAAAATCTTTGTCAGAGGCAGTTGGGATAACCAGCC11337451.3034164100223167No Hit
CTCCCAGTTGTAATTTTGTGAGCACCTAGTGAATCTAGAAGCAGAGCGAT11079031.2737070072308632No Hit
CTCTGAGAAGCATTCCATTCTGTAAAATTTCCCGGCATGTGCATCTTATG10904661.2536604606603738No Hit
CTATAGTTGAAGATGAGATTTAGGTGGGGACACAGCCAAACAATGTAAGA10887831.2517255901047661No Hit
CTCATCACACCTCTGTGGTTTCCCATAACATCTTCCGGCTTCACTTACAG10850211.2474005853334076No Hit
CTGTGCAGGAGTACATCATTACAGCAATCAAAGAAGTACTGAGGATTCAC10707621.2310076446011369No Hit
ATTGGTACTTGCAACACTTTCTCTGGAATTTGGCCATTTCTCTGCTCTTT10455201.2019880352341423No Hit
GTGATGTTTGGCACAATCGGATTGGTTCCCATGAGGTCCAGGGCTGCGGC10218941.1748262694903575No Hit
AGTGTGACATGCTCATAGAGACTTCCTCTTGAAGCATACAGAATGGAGAG10212451.1740801429362342No Hit
ATCTTCCAGAGATGCTGTCTGCATTAGCAAGCAAAATGTGCATATTTTTG10063741.1569836129110154No Hit
GTACGTTTGCTTTTGTGGTAATTTCTCTGGCCATGCTCTCCCTTCCCTCC9778591.1242011804135963No Hit
CCGTCTCTTGTATCAAATCAATTTTGGAATGAAGTGAGGATATAAACAAG9656491.1101638842258534No Hit
TAGAAGGGTCTATGCTGTGAAGATTGTTGTTTACCAGTTTTATGCACTCT9068361.0425491831046643No Hit
GGTCTAGAGTGATGGTGTCCTTCTCAAGCGTCTAAGTCCTGTCCAGGCTA9044791.0398394446022474No Hit
GTGATGCTTCTGCTGATTACCTGCTCCTCTGTGCACTCATGGTCACTTCT8947341.0286360497333242No Hit
ACTGCTTCTCAACTAGGAGGATTTTTGTCCCCTCTGGGTACGGGCAATGT8923331.0258757263797804No Hit
TATGGAAAATGTCAGACCCTTCCACAAAGTTAGAGTAGTGTAATGAACCA8522130.9797515393976146No Hit
GCTGAAGCACAATCACTTAGTATATTTCCTTACAGTCTTCTTTCAAATGC8447040.971118774690509No Hit
AACTTCGGTTGACAATGATCCTGCTCAGTGGTCTCCATCGTTTTTGCCAT8395700.9652164422885539No Hit
ATTGTGTGTCATCCAAAAGACAAACAGACTGGCCGAATAGAAAGCAGATC8353580.960374092448851No Hit
TGCATTGGCATGTTACATGATACTGTGTATAGGAGAAAGTGATGAAGAGA8205410.9433396438318333No Hit
GCTGTTTAGCAGTCTTCACTGTTGATGCCTTCAGGTACTGACAAATCTGG8192210.9418220982980232No Hit
AATGCTTGAAACTCCTGAACAGCCCTGGCTCCTCACACTTCCTGCCATCC7975060.9168573246111407No Hit
CTATGAATGTATTCTCCATCTAAAACTCAAGTGACTCTTAGAAAGGGATC7661810.8808443595758383No Hit
ATGATGCATGGAGGAACAGGTGGGAAGGTGGCCCAGTCACAGTCCCCACC7508730.8632454300064716No Hit
ATTGTTCCAAATGCTTCTTTCTTCCTGCCCTGCAATCCTCTGGGCCATAA7508020.863163804451244No Hit
GACTGACAGCAGCAATCTAGGAAATGGGACAATGAGTGAGATGAAAACTG7357490.8458580370872724No Hit
AAGAAGTTGCTGCTTGAAAATCCGGAGGTGGAGTTCAGTAATAAACTGAA7316520.8411478976539241No Hit
TATTCCTACTGTTGCTGCTGTTGTTATTTTCATGGTCATCCCACAGCCTC7275710.8364561527118947No Hit
GTACAAAGGGAAGGCATTAGCGTCTGAGGGAATGGAAAAACTCTTTGTAA7071050.8129272990035945No Hit
TGCTCAGCAACAATAGGATAAACTTATGAAAAGAAAGGAGTAGCAGTCCC6969110.8012077087220343No Hit
GTAACAGAACCAATCCATTGCCAAATATGCCTGTTCATTATTTGGTGAAC6944380.7983646101575554No Hit
CCTCAATGGAGAGAGAAAATTATCTGGAAGGCCTGAGTTCTCAGAGACTG6729680.7736815021189937No Hit
CAGTGTGTGGGTCATGGTTGGACCTCTGCTTGATGTGCTTTGAGAAGAAA6700270.7703003646834367No Hit
ACAGGAAAACCTTACCTAGCCAAACATCTGCATGGTGATACTGTGGATGT6595670.7582749659836996No Hit
GAGGAAACACAGGTGAACTTGGTTAGGGCACTGAGATGAGGTCTGCGTCT6589700.7575886215263626No Hit
AACATATTGCCACCTATCTGCTGGCTTCTTTCGTTGACATGAGGCAGTGG6184360.7109884770813203No Hit
CCAAAAAGGGAAGATTCTAGCAGCAAGGTCCCATTCTGACTCCAAGATGT5115100.5880603909084628No Hit
TCATACCGCTCTAGGTCCCAGGAAAGTAGGATCAAGTAGGCACTGCACTC5028440.5780974745478585No Hit
TTCTTTCCCTTCTGGTAATCACTTAGGGTTCTATGAGTAGCCACATGTTT4593750.5281230906015036No Hit
TTAGAGTCATAGGCCTTTCCCCCCAATCAGTCTTGAGGAAGAAGAAGAAA4540620.5220149698279183No Hit
TTTCAATATGGCTCTCTTTCTAGGCCATTGCGCATTGTGGCCGTGTGCCT4286780.49283210935046834No Hit
AAATGTCACACTAACTGGTGAATGTTTGAGATGGAGAACTGTTGGAGGAG4246020.48814611268697616No Hit
GCTGTTTAGCAGTCTTCACTGTTGATACCTTCAGGTGCTGGATAATCTGA4034730.46385503724464866No Hit
ACTCCTAAACTGTAAAGTTGCTGTTAGTTAGACCACCCCTGGCCCAAAGC2734780.31440554355704603No Hit
AAAACAATGGAGAAAGAAAAAAAAAGATTGTGTTTTTAGAGGATAAATTC2052550.23597258222892328No Hit
CCTGAATCCATTTACTGTGAAAATGAAAATGTTGAGGCTGTATTTCAACC1225180.14085351796313475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)