FastQCFastQC Report
Mon 6 Mar 2023
EGAF00002529165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86982563
Sequences flagged as poor quality0
Sequence length1-144
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATATCATTCTTGCCTGTGTACATACATCTCTTCTCCACTGCTCAGAAC12637961.4529302844295358No Hit
AGAAAGACTTGAGATTGAGGACTGGCCAACAGGGTCAAAATGCAAGGCCT12322111.4166184089103009No Hit
CTCCCAGTTGTAATTTTGTGAGCACCTAGTGAATCTAGAAGCAGAGCGAT10260551.1796099868889813No Hit
GATTATGGTCGCTGAAAAATCTTTGTCAGAGGCAGTTGGGATAACCAGCC10199881.1726350257119924No Hit
CTCTGAGAAGCATTCCATTCTGTAAAATTTCCCGGCATGTGCATCTTATG10198701.1724993663385155No Hit
CTCATCACACCTCTGTGGTTTCCCATAACATCTTCCGGCTTCACTTACAG10162261.1683100209406339No Hit
CTGTGCAGGAGTACATCATTACAGCAATCAAAGAAGTACTGAGGATTCAC10119091.1633469572516506No Hit
CTCTGGCTTGATCCTTGTATATGAGCCCTAGCATCCAGTCCAAGTATTTC9998941.1495338439268568No Hit
CTATAGTTGAAGATGAGATTTAGGTGGGGACACAGCCAAACAATGTAAGA9836581.1308680338609935No Hit
AGACATTTCAAAATGACCCTGTAGGCAAGGCCCAGACATAAAAGTTACAT9722161.1177136732565585No Hit
CCGTCTCTTGTATCAAATCAATTTTGGAATGAAGTGAGGATATAAACAAG9659611.1105225768065723No Hit
ATCTTCCAGAGATGCTGTCTGCATTAGCAAGCAAAATGTGCATATTTTTG9333951.0730828890383466No Hit
ATTGGTACTTGCAACACTTTCTCTGGAATTTGGCCATTTCTCTGCTCTTT9239011.0621680577519887No Hit
AGTGTGACATGCTCATAGAGACTTCCTCTTGAAGCATACAGAATGGAGAG9180141.0554000346023373No Hit
GTGATGTTTGGCACAATCGGATTGGTTCCCATGAGGTCCAGGGCTGCGGC9038551.0391220594408102No Hit
TAGAAGGGTCTATGCTGTGAAGATTGTTGTTTACCAGTTTTATGCACTCT8523690.979930885687974No Hit
GTACGTTTGCTTTTGTGGTAATTTCTCTGGCCATGCTCTCCCTTCCCTCC8442230.9705657902952343No Hit
GTGATGCTTCTGCTGATTACCTGCTCCTCTGTGCACTCATGGTCACTTCT8291790.9532703698326296No Hit
GGTCTAGAGTGATGGTGTCCTTCTCAAGCGTCTAAGTCCTGTCCAGGCTA8212680.9441754435311361No Hit
AACTTCGGTTGACAATGATCCTGCTCAGTGGTCTCCATCGTTTTTGCCAT8113070.9327237230294075No Hit
GCTGAAGCACAATCACTTAGTATATTTCCTTACAGTCTTCTTTCAAATGC7968560.91611004840131No Hit
TATGGAAAATGTCAGACCCTTCCACAAAGTTAGAGTAGTGTAATGAACCA7935800.9123437763037633No Hit
AATGCTTGAAACTCCTGAACAGCCCTGGCTCCTCACACTTCCTGCCATCC7790560.8956461768090233No Hit
TGCATTGGCATGTTACATGATACTGTGTATAGGAGAAAGTGATGAAGAGA7788210.8953760077177767No Hit
AAGAAGTTGCTGCTTGAAAATCCGGAGGTGGAGTTCAGTAATAAACTGAA7579060.8713309585968397No Hit
GCTGTTTAGCAGTCTTCACTGTTGATGCCTTCAGGTACTGACAAATCTGG7517720.8642789704874528No Hit
ACTGCTTCTCAACTAGGAGGATTTTTGTCCCCTCTGGGTACGGGCAATGT7465680.8582961621859774No Hit
ATTGTGTGTCATCCAAAAGACAAACAGACTGGCCGAATAGAAAGCAGATC7431680.8543873327807091No Hit
TATTCCTACTGTTGCTGCTGTTGTTATTTTCATGGTCATCCCACAGCCTC7025530.8076940662233647No Hit
GACTGACAGCAGCAATCTAGGAAATGGGACAATGAGTGAGATGAAAACTG6904980.7938349666702739No Hit
CTATGAATGTATTCTCCATCTAAAACTCAAGTGACTCTTAGAAAGGGATC6854280.7880062122335946No Hit
ATGATGCATGGAGGAACAGGTGGGAAGGTGGCCCAGTCACAGTCCCCACC6596900.7584163736357137No Hit
TGCTCAGCAACAATAGGATAAACTTATGAAAAGAAAGGAGTAGCAGTCCC6512660.7487316739563077No Hit
CAGTGTGTGGGTCATGGTTGGACCTCTGCTTGATGTGCTTTGAGAAGAAA6406990.7365832620958755No Hit
ACAGGAAAACCTTACCTAGCCAAACATCTGCATGGTGATACTGTGGATGT6390580.7346966770799798No Hit
ATTGTTCCAAATGCTTCTTTCTTCCTGCCCTGCAATCCTCTGGGCCATAA6344570.7294071111700858No Hit
GTACAAAGGGAAGGCATTAGCGTCTGAGGGAATGGAAAAACTCTTTGTAA6322970.726923854842033No Hit
GTAACAGAACCAATCCATTGCCAAATATGCCTGTTCATTATTTGGTGAAC6297300.7239726886410556No Hit
GAGGAAACACAGGTGAACTTGGTTAGGGCACTGAGATGAGGTCTGCGTCT6201620.7129727828323477No Hit
CCTCAATGGAGAGAGAAAATTATCTGGAAGGCCTGAGTTCTCAGAGACTG6180740.71057230171523No Hit
AACATATTGCCACCTATCTGCTGGCTTCTTTCGTTGACATGAGGCAGTGG5909120.6793453533899662No Hit
CCAAAAAGGGAAGATTCTAGCAGCAAGGTCCCATTCTGACTCCAAGATGT4759900.5472246201804838No Hit
TCATACCGCTCTAGGTCCCAGGAAAGTAGGATCAAGTAGGCACTGCACTC4622690.5314501942188115No Hit
TTCTTTCCCTTCTGGTAATCACTTAGGGTTCTATGAGTAGCCACATGTTT4577370.5262399545527303No Hit
TTAGAGTCATAGGCCTTTCCCCCCAATCAGTCTTGAGGAAGAAGAAGAAA4151340.4772611724490114No Hit
AAATGTCACACTAACTGGTGAATGTTTGAGATGGAGAACTGTTGGAGGAG4048350.4654208683181708No Hit
TTTCAATATGGCTCTCTTTCTAGGCCATTGCGCATTGTGGCCGTGTGCCT3878910.4459411020114457No Hit
GCTGTTTAGCAGTCTTCACTGTTGATACCTTCAGGTGCTGGATAATCTGA3696460.42496563362935164No Hit
ACTCCTAAACTGTAAAGTTGCTGTTAGTTAGACCACCCCTGGCCCAAAGC2503210.2877829663400468No Hit
AAAACAATGGAGAAAGAAAAAAAAAGATTGTGTTTTTAGAGGATAAATTC1972570.22677763588088337No Hit
CCTGAATCCATTTACTGTGAAAATGAAAATGTTGAGGCTGTATTTCAACC1163700.13378543467384377No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)