FastQCFastQC Report
Fri 10 May 2024
EGAF00002529166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6928994
Total Bases976.5 Mbp
Sequences flagged as poor quality0
Sequence length1-144
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACACGGTTTCTCCTATAATTAACATCTTTCATTTGCTACAATTGATGA4209316.074922275874392No Hit
GAGCTTTTATCATTTGGTGCTGCGGGTGACGCTGGCTCTGTGATCCTCTG3449154.977851041579774No Hit
AAAGGTTGAGTATGAAGGATCAGATAGATGTACGTATAAAACCAGACAAA3357144.845061202246676No Hit
GAGATAACGGAGAAATGTCTGAAATTTAGTTGATAGTAATATACAAATTT2847774.109932841621742No Hit
GAAATGTGTATGCTGTTTGCTAACATTATTATGTTTTCTTCTTTTTCTCC2828234.081732499696204No Hit
ATTCACTCTGCAAGTCTTCACCATGTGTCTGACAAGTGCATGGTTCTGTG2596653.7475137083391905No Hit
GAATCCAGGACAATGAGTTGGCCCAGCAGGAGGCAGGAACATGAGAAGGC2143813.0939700626093773No Hit
CCTTCTCCAACATACCCCACCTTGACAAATGTATAAATTATAAGCTTGGA1729882.496581754869466No Hit
CTACGCAGGCTATTTCCATTGGGCCTCCAGATCCACTTGCCAACCTCACT1714152.4738800466561237No Hit
AGAAAGCCTCAGCAGGGTGGCTGGTGCTGGGAGCCGGAATCTGCCCAGTT1488232.1478298292652585No Hit
TGGAGTGACAATATATGTGCATGTGTGTGCTTGTCTGTGTGTGTGAACAC1132451.6343642381563617No Hit
TGGAGTGACAATATATGTGCATGTGTGTGCTTGTGTGTGTGTGTGAACAC1117451.6127160739351196No Hit
CATATCCAGTGACAAGTATCACATTAAAAAACAACGAACAAAAGTAGTAT631200.9109547504298604No Hit
CCACAGAAGCGGTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCCCCTCCGC582500.8406703772582282No Hit
TTCAGTGATGACAGTGAGTTGTAAACTCAGTCTCTTTACATAATTGCATA574640.8293267392062974No Hit
TCAGTGATGACAGTGAGTTGTAAACTCAGTCTCTTTACATAATTGCATAT206100.2974457763998641No Hit
CCACAGAAGCGGTCTCTCTCTCTCTCTCTCTCTCTCTCCCCCCTCCGCTT186920.26976499041563606No Hit
CACACGGTTTCTCCTATAATTAACATCTTTCATTTGCTACAATTGATGAC103610.14953108633085843No Hit
TACGCAGGCTATTTCCATTGGGCCTCCAGATCCACTTGCCAACCTCACTA78200.1128590961400746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)