FastQCFastQC Report
Wed 8 May 2024
EGAF00002529167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6928994
Total Bases976.5 Mbp
Sequences flagged as poor quality0
Sequence length1-144
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACACGGTTTCTCCTATAATTAACATCTTTCATTTGCTACAATTGATGA3596015.189801001415213No Hit
GAGCTTTTATCATTTGGTGCTGCGGGTGACGCTGGCTCTGTGATCCTCTG2744793.9613109781881755No Hit
AAAGGTTGAGTATGAAGGATCAGATAGATGTACGTATAAAACCAGACAAA2592763.741899617751148No Hit
GAGATAACGGAGAAATGTCTGAAATTTAGTTGATAGTAATATACAAATTT2499933.6079263454406223No Hit
ATTCACTCTGCAAGTCTTCACCATGTGTCTGACAAGTGCATGGTTCTGTG2119403.0587412833666763No Hit
GAAATGTGTATGCTGTTTGCTAACATTATTATGTTTTCTTCTTTTTCTCC1901792.7446841489543794No Hit
GAATCCAGGACAATGAGTTGGCCCAGCAGGAGGCAGGAACATGAGAAGGC1697132.4493165963197545No Hit
CTACGCAGGCTATTTCCATTGGGCCTCCAGATCCACTTGCCAACCTCACT1409452.034133670775296No Hit
CCTTCTCCAACATACCCCACCTTGACAAATGTATAAATTATAAGCTTGGA1327011.9151553602153502No Hit
AGAAAGCCTCAGCAGGGTGGCTGGTGCTGGGAGCCGGAATCTGCCCAGTT1080161.558898737681112No Hit
TGGAGTGACAATATATGTGCATGTGTGTGCTTGTCTGTGTGTGTGAACAC1012741.4615974555613702No Hit
TGGAGTGACAATATATGTGCATGTGTGTGCTTGTGTGTGTGTGTGAACAC991811.4313910504179972No Hit
CATATCCAGTGACAAGTATCACATTAAAAAACAACGAACAAAAGTAGTAT595070.858811538875629No Hit
TTCAGTGATGACAGTGAGTTGTAAACTCAGTCTCTTTACATAATTGCATA506730.7313182837219948No Hit
CCACAGAAGCGGTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCCCCTCCGC412440.5952379234272681No Hit
TCAGTGATGACAGTGAGTTGTAAACTCAGTCTCTTTACATAATTGCATAT218810.3157889875499964No Hit
CCACAGAAGCGGTCTCTCTCTCTCTCTCTCTCTCTCTCCCCCCTCCGCTT132470.19118215429252788No Hit
CACACGGTTTCTCCTATAATTAACATCTTTCATTTGCTACAATTGATGAC99010.14289231596967758No Hit
AAGGTTGAGTATGAAGGATCAGATAGATGTACGTATAAAACCAGACAAAA77950.1124982934030539No Hit
GGAGTGACAATATATGTGCATGTGTGTGCTTGTCTGTGTGTGTGAACACA73350.10585952304187304No Hit
GGAGTGACAATATATGTGCATGTGTGTGCTTGTGTGTGTGTGTGAACACA72180.10417096623261617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)