FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002529168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6413380
Sequences flagged as poor quality0
Sequence length2-144
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACACGGTTTCTCCTATAATTAACATCTTTCATTTGCTACAATTGATGA3852286.006629889387499No Hit
AAAGGTTGAGTATGAAGGATCAGATAGATGTACGTATAAAACCAGACAAA3513355.478156603850076No Hit
CTACGCAGGCTATTTCCATTGGGCCTCCAGATCCACTTGCCAACCTCACT3349555.222753056890438No Hit
AGAAAGCCTCAGCAGGGTGGCTGGTGCTGGGAGCCGGAATCTGCCCAGTT3147754.908098381820507No Hit
GAGCTTTTATCATTTGGTGCTGCGGGTGACGCTGGCTCTGTGATCCTCTG3120394.865437569581094No Hit
GAAATGTGTATGCTGTTTGCTAACATTATTATGTTTTCTTCTTTTTCTCC3037424.7360674090729065No Hit
GAATCCAGGACAATGAGTTGGCCCAGCAGGAGGCAGGAACATGAGAAGGC1760572.7451515425563433No Hit
CCTTCTCCAACATACCCCACCTTGACAAATGTATAAATTATAAGCTTGGA1712102.6695751694114493No Hit
AAAGTTGCATGGTGCTGGAATCCGGTGAGGGGCTTCTTTCTTTATCCTCC479310.7473594266985583No Hit
TTCAGTGATGACAGTGAGTTGTTATAAAAACAAACCCAGCCCCTCATGCT320720.5000795212508848No Hit
TTAATTTTGATTTATGAAATGGAAGAATGTCATATACATGTTTGAAGCAA270030.42104163483217893No Hit
AAAGTTGCATGGTGCTGGTACCAAAACAGATATATAGACCAACTAAACAG205980.3211722991620643No Hit
AAAGGTTGAGTATGAAGGATCAATGACATTTCTTATTTTTATGAATTGAA184460.28761744976907655No Hit
AAAGGTTGAGTATGACGGATCAGATAGATGTACGTATAAAACCAGACAAA180630.28164555975164424No Hit
TGGAGTGACAATATATGTGCATGTGTGTGCTTGTCTGTGTGTGTGAACAC153110.23873526907808362No Hit
TGGAGTGACAATATATGTGCATGTGTGTGCTTGTGTGTGTGTGTGAACAC148290.23121973124935682No Hit
ATTCACTCTGCAAGTCTTCACCATGTGTCTGACAAGTGCATGGTTCTGTG140370.2188705487590007No Hit
AAAGGTTGAGTATGCAGGATCAGATAGATGTACGTATAAAACCAGACAAA136280.21249325628607693No Hit
AAAGTTGCATGGTGCTGGTGCTCTGCTGGTCCTCCCAGAGGCAGGAAAAC119010.18556517780016155No Hit
CCACACGGTTTCTCCTCTAATTAACATCTTTCATTTGCTACAATTGATGA114230.1781120095799719No Hit
GAGATAACGGAGAAATGTCTGAAATTTAGTTGATAGTAATATACAAATTT111960.1745725342954885No Hit
AGAAAGCCTCAGCCGGGTGGCTGGTGCTGGGAGCCGGAATCTGCCCAGTT97240.15162051835381654No Hit
TTCAGTGATGACAGTGAGTTGTAAACTCAGTCTCTTTACATAATTGCATA92390.14405820331868688No Hit
GAATCCAGGACAATGCGTTGGCCCAGCAGGAGGCAGGAACATGAGAAGGC78210.12194817709226648No Hit
CCTTCTCCAACATCCCCCACCTTGACAAATGTATAAATTATAAGCTTGGA66680.10397013743143242No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)