FastQCFastQC Report
Wed 8 May 2024
EGAF00002529169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6413380
Total Bases888.2 Mbp
Sequences flagged as poor quality0
Sequence length2-144
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACACGGTTTCTCCTATAATTAACATCTTTCATTTGCTACAATTGATGA3783175.898870798237435No Hit
AAAGGTTGAGTATGAAGGATCAGATAGATGTACGTATAAAACCAGACAAA3455235.387533562645594No Hit
CTACGCAGGCTATTTCCATTGGGCCTCCAGATCCACTTGCCAACCTCACT3130424.881076748921785No Hit
AGAAAGCCTCAGCAGGGTGGCTGGTGCTGGGAGCCGGAATCTGCCCAGTT2991044.6637498479740795No Hit
GAGCTTTTATCATTTGGTGCTGCGGGTGACGCTGGCTCTGTGATCCTCTG2967784.627481920609725No Hit
GAAATGTGTATGCTGTTTGCTAACATTATTATGTTTTCTTCTTTTTCTCC2583204.027829319329277No Hit
GAATCCAGGACAATGAGTTGGCCCAGCAGGAGGCAGGAACATGAGAAGGC1624172.5324711774446547No Hit
CCTTCTCCAACATACCCCACCTTGACAAATGTATAAATTATAAGCTTGGA1594812.486691884778385No Hit
AAAGTTGCATGGTGCTGGAATCCGGTGAGGGGCTTCTTTCTTTATCCTCC433020.6751821972189391No Hit
TTCAGTGATGACAGTGAGTTGTTATAAAAACAAACCCAGCCCCTCATGCT334760.5219712538474253No Hit
TTAATTTTGATTTATGAAATGGAAGAATGTCATATACATGTTTGAAGCAA303560.47332295918844663No Hit
AAAGTTGCATGGTGCTGGTACCAAAACAGATATATAGACCAACTAAACAG183980.2868690144666307No Hit
AAAGGTTGAGTATGACGGATCAGATAGATGTACGTATAAAACCAGACAAA178880.2789168893781438No Hit
AAAGGTTGAGTATGAAGGATCAATGACATTTCTTATTTTTATGAATTGAA167280.26082970290236973No Hit
TGGAGTGACAATATATGTGCATGTGTGTGCTTGTCTGTGTGTGTGAACAC153300.2390315247186351No Hit
TGGAGTGACAATATATGTGCATGTGTGTGCTTGTGTGTGTGTGTGAACAC150160.23413551044846867No Hit
AAAGGTTGAGTATGCAGGATCAGATAGATGTACGTATAAAACCAGACAAA138420.21583003034281456No Hit
ATTCACTCTGCAAGTCTTCACCATGTGTCTGACAAGTGCATGGTTCTGTG123020.19181773105601102No Hit
GAGATAACGGAGAAATGTCTGAAATTTAGTTGATAGTAATATACAAATTT107400.16746239892225318No Hit
AGAAAGCCTCAGCCGGGTGGCTGGTGCTGGGAGCCGGAATCTGCCCAGTT97140.15146459433247367No Hit
AAAGTTGCATGGTGCTGGTGCTCTGCTGGTCCTCCCAGAGGCAGGAAAAC96900.15109037668125078No Hit
TTCAGTGATGACAGTGAGTTGTAAACTCAGTCTCTTTACATAATTGCATA95040.14819018988427318No Hit
CCACCCGGTTTCTCCTATAATTAACATCTTTCATTTGCTACAATTGATGA79070.12328912367581524No Hit
GAATCCAGGACAATGCGTTGGCCCAGCAGGAGGCAGGAACATGAGAAGGC70380.10973932622111897No Hit
CCACACGGTTTCTCCTCTAATTAACATCTTTCATTTGCTACAATTGATGA65580.10225497319666073No Hit
CTACGCCGGCTATTTCCATTGGGCCTCCAGATCCACTTGCCAACCTCACT64490.10055540136402333No Hit
AAGGTTGAGTATGAAGGATCAGATAGATGTACGTATAAAACCAGACAAAA64180.1000720368978604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)