FastQCFastQC Report
Fri 6 Jan 2023
EGAF00002529170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197378229
Sequences flagged as poor quality0
Sequence length1-144
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACACGGTTTCTCCTATAATTAACATCTTTCATTTGCTACAATTGATGA167345458.478414810379112No Hit
AAAGGTTGAGTATGAAGGATCAGATAGATGTACGTATAAAACCAGACAAA141435527.165710256727453No Hit
GAAATGTGTATGCTGTTTGCTAACATTATTATGTTTTCTTCTTTTTCTCC102374325.186707800483912No Hit
GAGCTTTTATCATTTGGTGCTGCGGGTGACGCTGGCTCTGTGATCCTCTG73026593.699830035459483No Hit
CCTTCTCCAACATACCCCACCTTGACAAATGTATAAATTATAAGCTTGGA71421733.618521169323087No Hit
CTACGCAGGCTATTTCCATTGGGCCTCCAGATCCACTTGCCAACCTCACT70695763.5817405170861063No Hit
AGAAAGCCTCAGCAGGGTGGCTGGTGCTGGGAGCCGGAATCTGCCCAGTT64305773.2579971117280624No Hit
GAATCCAGGACAATGAGTTGGCCCAGCAGGAGGCAGGAACATGAGAAGGC46479602.3548493790568967No Hit
TTAATTTTGATTTATGAAATGGAAGAATGTCATATACATGTTTGAAGCAA13460770.6819784567020307No Hit
AAAGTTGCATGGTGCTGGAATCCGGTGAGGGGCTTCTTTCTTTATCCTCC4566150.23134010387741394No Hit
TGGAGTGACAATATATGTGCATGTGTGTGCTTGTCTGTGTGTGTGAACAC4197770.21267644467516225No Hit
TGGAGTGACAATATATGTGCATGTGTGTGCTTGTGTGTGTGTGTGAACAC4113080.20838569789781627No Hit
TTCAGTGATGACAGTGAGTTGTTATAAAAACAAACCCAGCCCCTCATGCT4029800.20416638757053598No Hit
ATTCACTCTGCAAGTCTTCACCATGTGTCTGACAAGTGCATGGTTCTGTG2856600.1447272079840173No Hit
GAGATAACGGAGAAATGTCTGAAATTTAGTTGATAGTAATATACAAATTT2217560.11235079021810455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)