FastQCFastQC Report
Fri 6 Jan 2023
EGAF00002529171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197378229
Sequences flagged as poor quality0
Sequence length1-144
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACACGGTTTCTCCTATAATTAACATCTTTCATTTGCTACAATTGATGA163897218.303712665290963No Hit
AAAGGTTGAGTATGAAGGATCAGATAGATGTACGTATAAAACCAGACAAA139818877.08380406027455No Hit
GAAATGTGTATGCTGTTTGCTAACATTATTATGTTTTCTTCTTTTTCTCC102669075.201641058396567No Hit
GAGCTTTTATCATTTGGTGCTGCGGGTGACGCTGGCTCTGTGATCCTCTG71897643.6426327444654496No Hit
CCTTCTCCAACATACCCCACCTTGACAAATGTATAAATTATAAGCTTGGA68434423.467171650425539No Hit
CTACGCAGGCTATTTCCATTGGGCCTCCAGATCCACTTGCCAACCTCACT67773443.4336836612309454No Hit
AGAAAGCCTCAGCAGGGTGGCTGGTGCTGGGAGCCGGAATCTGCCCAGTT61010773.0910587408300234No Hit
GAATCCAGGACAATGAGTTGGCCCAGCAGGAGGCAGGAACATGAGAAGGC43446252.201167282740185No Hit
TTAATTTTGATTTATGAAATGGAAGAATGTCATATACATGTTTGAAGCAA14247440.7218344227822614No Hit
TTCAGTGATGACAGTGAGTTGTTATAAAAACAAACCCAGCCCCTCATGCT4350830.22043109931845625No Hit
AAAGTTGCATGGTGCTGGAATCCGGTGAGGGGCTTCTTTCTTTATCCTCC4304420.21807977616416854No Hit
TGGAGTGACAATATATGTGCATGTGTGTGCTTGTCTGTGTGTGTGAACAC4199860.21278233274653607No Hit
TGGAGTGACAATATATGTGCATGTGTGTGCTTGTGTGTGTGTGTGAACAC4098490.2076465079641585No Hit
ATTCACTCTGCAAGTCTTCACCATGTGTCTGACAAGTGCATGGTTCTGTG2544340.12890682082267543No Hit
GAGATAACGGAGAAATGTCTGAAATTTAGTTGATAGTAATATACAAATTT2152640.10906167366614683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)