FastQCFastQC Report
Wed 22 May 2024
EGAF00002529172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168427707
Total Bases23.8 Gbp
Sequences flagged as poor quality0
Sequence length1-144
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACACGGTTTCTCCTATAATTAACATCTTTCATTTGCTACAATTGATGA114374236.790701603507551No Hit
AAAGGTTGAGTATGAAGGATCAGATAGATGTACGTATAAAACCAGACAAA84204354.999435751981116No Hit
CTACGCAGGCTATTTCCATTGGGCCTCCAGATCCACTTGCCAACCTCACT75801034.5005083397590875No Hit
CCTTCTCCAACATACCCCACCTTGACAAATGTATAAATTATAAGCTTGGA72855664.325633905352639No Hit
AGAAAGCCTCAGCAGGGTGGCTGGTGCTGGGAGCCGGAATCTGCCCAGTT64842963.849898639301668No Hit
GAGCTTTTATCATTTGGTGCTGCGGGTGACGCTGGCTCTGTGATCCTCTG63421753.765517629471735No Hit
ATTCACTCTGCAAGTCTTCACCATGTGTCTGACAAGTGCATGGTTCTGTG51132053.035845521544742No Hit
GAAATGTGTATGCTGTTTGCTAACATTATTATGTTTTCTTCTTTTTCTCC50184252.979572119924425No Hit
GAATCCAGGACAATGAGTTGGCCCAGCAGGAGGCAGGAACATGAGAAGGC47115932.7973978176880365No Hit
GAGATAACGGAGAAATGTCTGAAATTTAGTTGATAGTAATATACAAATTT28282051.679180373808687No Hit
CATATCCAGTGACAAGTATCACATTAAAAAACAACGAACAAAAGTAGTAT23479901.3940639825964025No Hit
TTCAGTGATGACAGTGAGTTGTAAACTCAGTCTCTTTACATAATTGCATA22012301.306928675339622No Hit
TGGAGTGACAATATATGTGCATGTGTGTGCTTGTCTGTGTGTGTGAACAC20881211.239772859936875No Hit
TGGAGTGACAATATATGTGCATGTGTGTGCTTGTGTGTGTGTGTGAACAC20540761.2195594398254201No Hit
CCACAGAAGCGGTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCCCCTCCGC9141790.5427723361453826No Hit
CCACAGAAGCGGTCTCTCTCTCTCTCTCTCTCTCTCTCCCCCCTCCGCTT2670530.15855645413494823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)