FastQCFastQC Report
Fri 10 Feb 2023
EGAF00002529173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168427707
Sequences flagged as poor quality0
Sequence length1-144
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACACGGTTTCTCCTATAATTAACATCTTTCATTTGCTACAATTGATGA115190206.839147908128916No Hit
AAAGGTTGAGTATGAAGGATCAGATAGATGTACGTATAAAACCAGACAAA86119775.113159321227356No Hit
CTACGCAGGCTATTTCCATTGGGCCTCCAGATCCACTTGCCAACCTCACT76733464.555869183684844No Hit
CCTTCTCCAACATACCCCACCTTGACAAATGTATAAATTATAAGCTTGGA71602884.251253031664202No Hit
AGAAAGCCTCAGCAGGGTGGCTGGTGCTGGGAGCCGGAATCTGCCCAGTT64069963.804003577629897No Hit
GAGCTTTTATCATTTGGTGCTGCGGGTGACGCTGGCTCTGTGATCCTCTG64062673.8035707509810126No Hit
GAAATGTGTATGCTGTTTGCTAACATTATTATGTTTTCTTCTTTTTCTCC51112163.0346645994533428No Hit
ATTCACTCTGCAAGTCTTCACCATGTGTCTGACAAGTGCATGGTTCTGTG49254112.9243472393767136No Hit
GAATCCAGGACAATGAGTTGGCCCAGCAGGAGGCAGGAACATGAGAAGGC45579182.7061568913955467No Hit
GAGATAACGGAGAAATGTCTGAAATTTAGTTGATAGTAATATACAAATTT28678591.702724006092418No Hit
CATATCCAGTGACAAGTATCACATTAAAAAACAACGAACAAAAGTAGTAT23203511.3776539747109424No Hit
TTCAGTGATGACAGTGAGTTGTAAACTCAGTCTCTTTACATAATTGCATA22250141.3210498674069107No Hit
TGGAGTGACAATATATGTGCATGTGTGTGCTTGTCTGTGTGTGTGAACAC21534311.278549140373917No Hit
TGGAGTGACAATATATGTGCATGTGTGTGCTTGTGTGTGTGTGTGAACAC21043351.2493995420836548No Hit
CCACAGAAGCGGTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCCCCTCCGC8505160.5049739233224851No Hit
CCACAGAAGCGGTCTCTCTCTCTCTCTCTCTCTCTCTCCCCCCTCCGCTT2501760.14853613129103516No Hit
AAGGTTGAGTATGAAGGATCAGATAGATGTACGTATAAAACCAGACAAAA1781170.10575279042420258No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)