FastQCFastQC Report
Thu 9 May 2024
EGAF00002529174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3080376
Total Bases435.4 Mbp
Sequences flagged as poor quality0
Sequence length1-144
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGTCTAAGTATCCTGGGCATAGCAACTAAACTTTTGTATATTTCTTC2082466.760408469615397No Hit
AAGTAAGATATGGGCAGGAATTTACGTTTGCTTTTTGTTATCTTTCAAGT1542035.005979789480245No Hit
CTAAACCCTTAACCCTGCTACCCTAATTCCCAGTACCCAAGATCCCCTAA1531384.971406088087948No Hit
TACTCTAAAAACATTGCCCAAAGTAGGGAAATTCGGGTTTAGTATTTTTT1492414.844895558204583No Hit
TAGAACTGTCTTGGACATCGTGACCAAATAGACAAAATCTGTTTGAAATG1321054.288599833267108No Hit
AATTGCTATTGCTCAGTTTGGGATTCACTAAGAATTGCAACACTATATCC1163273.7763896355509847No Hit
GGATGCGTACAATTCTTAAATCACTTCCCTGGCCAAAGAAACTCTAGCCC1015773.297551987160009No Hit
AATGAAAAGTCCTCTGCAGTTGGACTTTACTTGTTATTTTCCACAGATTT861402.796411866603298No Hit
CCCATCAATTTCACAGTAAAGAAGCTGAGGCCCAGAGAGGTGAGGAGATT835452.7121689040558685No Hit
ATTTTCCAAATGCTAAACATCATATTTATGGATTATTCAGGGTTATATTA504861.6389557638418168No Hit
ATTTTTAGGCAAAATCTTCCTTTTGCTTGAATAAATGATACAAGAGGCAG502811.6323007321184166No Hit
TCACAGGAAAGCTAGATTTGATTGCTCTTTCAGGAAAGCTGGTGGGAGCT498371.6178869073126139No Hit
GACCGCGACAACAACAAGTGTGCCTTCTGTACCCACAGGGGCAAGTACTG248300.806070427765961No Hit
GACCGCGACAACAACAAGTGTGCCTTCTGTACCCACAGGGGCAAGTCCTG195590.634954953551125No Hit
AATGAAAAGTCCTCTGCAGTCCACCACCGAGCCGCCGGTTCCTATTCTGT164450.5338633984942098No Hit
GAAAATGGTTCCCTTTTTATTTTATTTAAGAGTGAGATGGAAGCTGCATA91130.29584050778216686No Hit
AAAGTTGCATGGTGCTGGTGCTGATAAGGGAAGCCACAAGGAGACGATCG59120.19192462218897952No Hit
AAAGTTGCATGGTGCTGGTGCTGGCAAAGGTGGCCAGGTCTCGGGGGGCT56400.1830945313169561No Hit
CT34180.11096048014917659No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)