FastQCFastQC Report
Wed 8 May 2024
EGAF00002529175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3080376
Total Bases435.4 Mbp
Sequences flagged as poor quality0
Sequence length1-144
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGTCTAAGTATCCTGGGCATAGCAACTAAACTTTTGTATATTTCTTC1933736.277577802190382No Hit
CTAAACCCTTAACCCTGCTACCCTAATTCCCAGTACCCAAGATCCCCTAA1405014.561163961802066No Hit
TACTCTAAAAACATTGCCCAAAGTAGGGAAATTCGGGTTTAGTATTTTTT1393794.524739836954969No Hit
AAGTAAGATATGGGCAGGAATTTACGTTTGCTTTTTGTTATCTTTCAAGT1381504.484842110183951No Hit
TAGAACTGTCTTGGACATCGTGACCAAATAGACAAAATCTGTTTGAAATG1244514.04012367321392No Hit
AATTGCTATTGCTCAGTTTGGGATTCACTAAGAATTGCAACACTATATCC1037433.3678680784423722No Hit
GGATGCGTACAATTCTTAAATCACTTCCCTGGCCAAAGAAACTCTAGCCC906372.942400538116126No Hit
AATGAAAAGTCCTCTGCAGTTGGACTTTACTTGTTATTTTCCACAGATTT788232.5588759294319914No Hit
CCCATCAATTTCACAGTAAAGAAGCTGAGGCCCAGAGAGGTGAGGAGATT762152.4742109404825903No Hit
TCACAGGAAAGCTAGATTTGATTGCTCTTTCAGGAAAGCTGGTGGGAGCT478511.5534142585190898No Hit
ATTTTCCAAATGCTAAACATCATATTTATGGATTATTCAGGGTTATATTA470201.5264370323622831No Hit
ATTTTTAGGCAAAATCTTCCTTTTGCTTGAATAAATGATACAAGAGGCAG443891.441025381317086No Hit
GACCGCGACAACAACAAGTGTGCCTTCTGTACCCACAGGGGCAAGTACTG231750.7523432204380245No Hit
GACCGCGACAACAACAAGTGTGCCTTCTGTACCCACAGGGGCAAGTCCTG183720.5964206966941699No Hit
AATGAAAAGTCCTCTGCAGTCCACCACCGAGCCGCCGGTTCCTATTCTGT131880.4281295530156059No Hit
GAAAATGGTTCCCTTTTTATTTTATTTAAGAGTGAGATGGAAGCTGCATA87800.28503013917781467No Hit
NN84750.27512875051617075No Hit
AAAGTTGCATGGTGCTGGTGCTGATAAGGGAAGCCACAAGGAGACGATCG49260.15991554277789466No Hit
AAAGTTGCATGGTGCTGGTGCTGGCAAAGGTGGCCAGGTCTCGGGGGGCT48280.1567341129784156No Hit
N44420.14420317519679415No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)