FastQCFastQC Report
Fri 10 May 2024
EGAF00002529176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6451545
Total Bases913.1 Mbp
Sequences flagged as poor quality0
Sequence length1-144
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGTCTAAGTATCCTGGGCATAGCAACTAAACTTTTGTATATTTCTTC3163524.90350760941759No Hit
CTAAACCCTTAACCCTGCTACCCTAATTCCCAGTACCCAAGATCCCCTAA2934954.549220380544505No Hit
TACTCTAAAAACATTGCCCAAAGTAGGGAAATTCGGGTTTAGTATTTTTT2879734.463628479689749No Hit
AAGTAAGATATGGGCAGGAATTTACGTTTGCTTTTTGTTATCTTTCAAGT2348943.640895320423247No Hit
CCCATCAATTTCACAGTAAAGAAGCTGAGGCCCAGAGAGGTGAGGAGATT2215353.433828641046447No Hit
ATTTTCCAAATGCTAAACATCATATTTATGGATTATTCAGGGTTATATTA2183513.3844761216111796No Hit
AATTGCTATTGCTCAGTTTGGGATTCACTAAGAATTGCAACACTATATCC2136243.311206850452101No Hit
TAGAACTGTCTTGGACATCGTGACCAAATAGACAAAATCTGTTTGAAATG2042113.165303814822651No Hit
ATTTTTAGGCAAAATCTTCCTTTTGCTTGAATAAATGATACAAGAGGCAG1896202.9391409344583352No Hit
AATGAAAAGTCCTCTGCAGTTGGACTTTACTTGTTATTTTCCACAGATTT1863332.8881918982197288No Hit
GGATGCGTACAATTCTTAAATCACTTCCCTGGCCAAAGAAACTCTAGCCC1792282.7780632391155917No Hit
TCACAGGAAAGCTAGATTTGATTGCTCTTTCAGGAAAGCTGGTGGGAGCT1139091.7656080830250738No Hit
GAAAATGGTTCCCTTTTTATTTTATTTAAGAGTGAGATGGAAGCTGCATA1131961.7545564667068123No Hit
GACCGCGACAACAACAAGTGTGCCTTCTGTACCCACAGGGGCAAGTACTG843551.3075162616086533No Hit
AATGAAAAGTCCTCTGCAGTCCACCACCGAGCCGCCGGTTCCTATTCTGT475160.7365057517230369No Hit
AAAGTTGCATGGTGCTGGTGCTGATAAGGGAAGCCACAAGGAGACGATCG246910.38271452807040796No Hit
AAAGTTGCATGGTGCTGGTGCTGGCAAAGGTGGCCAGGTCTCGGGGGGCT245200.3800640001736018No Hit
GACCGCGACAACAACAAGTGTGCCTTCTGTACCCACAGGGGCAAGTCCTG181480.28129696065051085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)