FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002529177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6451545
Sequences flagged as poor quality0
Sequence length1-144
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGTCTAAGTATCCTGGGCATAGCAACTAAACTTTTGTATATTTCTTC2914124.516933540725516No Hit
TACTCTAAAAACATTGCCCAAAGTAGGGAAATTCGGGTTTAGTATTTTTT2718664.21396735200638No Hit
CTAAACCCTTAACCCTGCTACCCTAATTCCCAGTACCCAAGATCCCCTAA2675944.147750655075645No Hit
AAGTAAGATATGGGCAGGAATTTACGTTTGCTTTTTGTTATCTTTCAAGT2090153.23976659854345No Hit
ATTTTCCAAATGCTAAACATCATATTTATGGATTATTCAGGGTTATATTA2009763.1151607870672837No Hit
CCCATCAATTTCACAGTAAAGAAGCTGAGGCCCAGAGAGGTGAGGAGATT1991643.0870744914590227No Hit
TAGAACTGTCTTGGACATCGTGACCAAATAGACAAAATCTGTTTGAAATG1926592.986245930238416No Hit
AATTGCTATTGCTCAGTTTGGGATTCACTAAGAATTGCAACACTATATCC1888392.9270353070466064No Hit
ATTTTTAGGCAAAATCTTCCTTTTGCTTGAATAAATGATACAAGAGGCAG1714902.65812297674433No Hit
AATGAAAAGTCCTCTGCAGTTGGACTTTACTTGTTATTTTCCACAGATTT1698822.6331987144164692No Hit
GGATGCGTACAATTCTTAAATCACTTCCCTGGCCAAAGAAACTCTAGCCC1546382.396914227522245No Hit
TCACAGGAAAGCTAGATTTGATTGCTCTTTCAGGAAAGCTGGTGGGAGCT1122221.7394593078092146No Hit
GAAAATGGTTCCCTTTTTATTTTATTTAAGAGTGAGATGGAAGCTGCATA1086771.6845112294806903No Hit
GACCGCGACAACAACAAGTGTGCCTTCTGTACCCACAGGGGCAAGTACTG796671.234851496812004No Hit
AATGAAAAGTCCTCTGCAGTCCACCACCGAGCCGCCGGTTCCTATTCTGT394690.6117759389417573No Hit
AAAGTTGCATGGTGCTGGTGCTGATAAGGGAAGCCACAAGGAGACGATCG218800.33914356948606883No Hit
AAAGTTGCATGGTGCTGGTGCTGGCAAAGGTGGCCAGGTCTCGGGGGGCT208210.322728896721638No Hit
GACCGCGACAACAACAAGTGTGCCTTCTGTACCCACAGGGGCAAGTCCTG171960.2665408053419762No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)