FastQCFastQC Report
Tue 24 Jan 2023
EGAF00002529179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187545161
Sequences flagged as poor quality0
Sequence length1-144
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGGACCACAACTGTGTAGCTCACACTTAGCTGGCTCAATGAATATCA132129707.045220430933965No Hit
AGGCTGTCTTTGGTTTCTTAAGCCAGCTAAAACTTTAGGCAGGCTTAGTT113689376.061972987935423No Hit
CACAATCAAAGGCTAACTTGTGAAAATTTTATTCTTCTACCTTACAGAAT90611074.831426709004772No Hit
AAAACTTTGCTAATCCTCAAACTTAAAAGTGATTCAACTTTAGGAATAGC87713444.67692365573751No Hit
CAAGTGTGGCTGATTTTAATTGATTTCTGCTTGGTACACATTTACTGATG85228114.544404640757433No Hit
ACCTAGGTGATCCTTTCAACTCCCAGGAATCAGCAATTCCATAACTAAGG63129703.3661065773912453No Hit
TTTCTTTACCTGTCACCACACATTCAGGAGTTCCTGATTCATCTCTTAGA54867782.925576949436728No Hit
TACAGGAGCTGAAATTAGGAACATGAAAGAAACTTGAAGAGAGAAGACAT46203812.4636098182239956No Hit
TCCTTTGGAGTAATGTTAAGCATATACTGGCACCCACAGTAATATTTTAA38618632.059164299099138No Hit
GTGGACATGTTCTATTCTGCCGCCAGCCACTCCTGCCCTTGCCCAATGGA33230251.7718532337925796No Hit
GAGAGGGATAGTTCTTCCAAGCTCCACAAAGCCCCAAGATATAAGAGTAT29089951.551090406432827No Hit
TGTGCTTTGTAAAATGTGTGGCAGTATCCCTGGACTCTACCACCAGATGC26857681.4320646748118444No Hit
TGGATTTTCTATTTGGAATATTTTAAAAAGAATCAAATCAAAACAAAATA6757060.3602897544234692No Hit
CAGGAGCTGAAATTAGGAACATGAAAGAAACTTGAAGAGAGAAGACATTC1917610.10224790603901532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)