FastQCFastQC Report
Sun 19 May 2024
EGAF00002529179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187545161
Total Bases26.7 Gbp
Sequences flagged as poor quality0
Sequence length1-144
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGGACCACAACTGTGTAGCTCACACTTAGCTGGCTCAATGAATATCA132130697.04527321821969No Hit
AGGCTGTCTTTGGTTTCTTAAGCCAGCTAAAACTTTAGGCAGGCTTAGTT113689666.061988450877706No Hit
CACAATCAAAGGCTAACTTGTGAAAATTTTATTCTTCTACCTTACAGAAT90611224.831434707078365No Hit
AAAACTTTGCTAATCCTCAAACTTAAAAGTGATTCAACTTTAGGAATAGC87713744.6769396518847No Hit
CAAGTGTGGCTGATTTTAATTGATTTCTGCTTGGTACACATTTACTGATG85228264.544412638831028No Hit
ACCTAGGTGATCCTTTCAACTCCCAGGAATCAGCAATTCCATAACTAAGG63129983.3661215071286215No Hit
TTTCTTTACCTGTCACCACACATTCAGGAGTTCCTGATTCATCTCTTAGA54867952.9255860139201353No Hit
TACAGGAGCTGAAATTAGGAACATGAAAGAAACTTGAAGAGAGAAGACAT46203852.463611951043621No Hit
TCCTTTGGAGTAATGTTAAGCATATACTGGCACCCACAGTAATATTTTAA38618702.059168031533482No Hit
GTGGACATGTTCTATTCTGCCGCCAGCCACTCCTGCCCTTGCCCAATGGA33230291.7718553666122048No Hit
GAGAGGGATAGTTCTTCCAAGCTCCACAAAGCCCCAAGATATAAGAGTAT29089981.5510920060475462No Hit
TGTGCTTTGTAAAATGTGTGGCAGTATCCCTGGACTCTACCACCAGATGC26857781.4320700068609076No Hit
TGGATTTTCTATTTGGAATATTTTAAAAAGAATCAAATCAAAACAAAATA6757070.36029028762837556No Hit
CAGGAGCTGAAATTAGGAACATGAAAGAAACTTGAAGAGAGAAGACATTC1917620.10224843924392163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)