FastQCFastQC Report
Fri 6 Jan 2023
EGAF00002529180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216441624
Sequences flagged as poor quality0
Sequence length1-144
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGTCTAAGTATCCTGGGCATAGCAACTAAACTTTTGTATATTTCTTC111169895.136252812444246No Hit
AATTGCTATTGCTCAGTTTGGGATTCACTAAGAATTGCAACACTATATCC102059484.7153351612257355No Hit
AAGTAAGATATGGGCAGGAATTTACGTTTGCTTTTTGTTATCTTTCAAGT93210664.306503447784147No Hit
TAGAACTGTCTTGGACATCGTGACCAAATAGACAAAATCTGTTTGAAATG86222723.983647803344887No Hit
TACTCTAAAAACATTGCCCAAAGTAGGGAAATTCGGGTTTAGTATTTTTT80680003.727563973554366No Hit
CCCATCAATTTCACAGTAAAGAAGCTGAGGCCCAGAGAGGTGAGGAGATT76142353.517916221142381No Hit
GGATGCGTACAATTCTTAAATCACTTCCCTGGCCAAAGAAACTCTAGCCC68365293.158601785394107No Hit
CTAAACCCTTAACCCTGCTACCCTAATTCCCAGTACCCAAGATCCCCTAA68302033.155679057370222No Hit
AATGAAAAGTCCTCTGCAGTTGGACTTTACTTGTTATTTTCCACAGATTT64979883.002189634282175No Hit
ATTTTTAGGCAAAATCTTCCTTTTGCTTGAATAAATGATACAAGAGGCAG55344422.5570137100800907No Hit
ATTTTCCAAATGCTAAACATCATATTTATGGATTATTCAGGGTTATATTA53755172.4835874452688453No Hit
TCACAGGAAAGCTAGATTTGATTGCTCTTTCAGGAAAGCTGGTGGGAGCT38584311.782665888701704No Hit
GAAAATGGTTCCCTTTTTATTTTATTTAAGAGTGAGATGGAAGCTGCATA29909671.3818816107201266No Hit
GACCGCGACAACAACAAGTGTGCCTTCTGTACCCACAGGGGCAAGTACTG26308411.21549679372208No Hit
AATGAAAAGTCCTCTGCAGTCCACCACCGAGCCGCCGGTTCCTATTCTGT15596960.7206081580685239No Hit
TACTCTAAAAACATTGCCCACATAGGTATGAATGTCAGAGGTGTGTGAAC8413990.38874176992868986No Hit
AAAGTTGCATGGTGCTGGTGGGAGTGTAGCAGTGAGGATGACCAGAGGTT8137480.3759665007872977No Hit
AAAGTTGCATGGTGCTGGTGGGAGTGTAGCAGTGAGGACAACCAGAGGTC4928130.22768864458344668No Hit
GACCGCGACAACAACAACAAAGCAAAACAAAACAAAACAAACAAACAAAC4079290.18847067974319026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)