FastQCFastQC Report
Mon 23 Jan 2023
EGAF00002529181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216441624
Sequences flagged as poor quality0
Sequence length1-144
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGTCTAAGTATCCTGGGCATAGCAACTAAACTTTTGTATATTTCTTC108408725.008681694238258No Hit
AATTGCTATTGCTCAGTTTGGGATTCACTAAGAATTGCAACACTATATCC96207544.444964800301073No Hit
AAGTAAGATATGGGCAGGAATTTACGTTTGCTTTTTGTTATCTTTCAAGT90100304.16279911113585No Hit
TAGAACTGTCTTGGACATCGTGACCAAATAGACAAAATCTGTTTGAAATG85578833.9538989043992756No Hit
TACTCTAAAAACATTGCCCAAAGTAGGGAAATTCGGGTTTAGTATTTTTT77922773.600174890574652No Hit
CCCATCAATTTCACAGTAAAGAAGCTGAGGCCCAGAGAGGTGAGGAGATT70005693.234391274018532No Hit
CTAAACCCTTAACCCTGCTACCCTAATTCCCAGTACCCAAGATCCCCTAA64405152.9756360541815194No Hit
AATGAAAAGTCCTCTGCAGTTGGACTTTACTTGTTATTTTCCACAGATTT58836172.7183389642280638No Hit
GGATGCGTACAATTCTTAAATCACTTCCCTGGCCAAAGAAACTCTAGCCC58831722.7181333660664087No Hit
ATTTTCCAAATGCTAAACATCATATTTATGGATTATTCAGGGTTATATTA50731082.343868940846609No Hit
ATTTTTAGGCAAAATCTTCCTTTTGCTTGAATAAATGATACAAGAGGCAG40230571.8587261200738356No Hit
TCACAGGAAAGCTAGATTTGATTGCTCTTTCAGGAAAGCTGGTGGGAGCT38575361.78225238228669No Hit
GAAAATGGTTCCCTTTTTATTTTATTTAAGAGTGAGATGGAAGCTGCATA28366981.3106065033036345No Hit
GACCGCGACAACAACAAGTGTGCCTTCTGTACCCACAGGGGCAAGTACTG25165351.162685325258879No Hit
AATGAAAAGTCCTCTGCAGTCCACCACCGAGCCGCCGGTTCCTATTCTGT13513720.624358649240222No Hit
TACTCTAAAAACATTGCCCACATAGGTATGAATGTCAGAGGTGTGTGAAC8071350.37291117349960373No Hit
AAAGTTGCATGGTGCTGGTGGGAGTGTAGCAGTGAGGATGACCAGAGGTT8049900.3719201441585931No Hit
AAAGTTGCATGGTGCTGGTGGGAGTGTAGCAGTGAGGACAACCAGAGGTC4521410.20889743462653007No Hit
GACCGCGACAACAACAACAAAGCAAAACAAAACAAAACAAACAAACAAAC4232820.19556404732945454No Hit
AAAGTTGCATGGTGCTGGTGGGAGTGTAGCAGTGAGGACGACCGGAGGTC2200600.10167175607590155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)