FastQCFastQC Report
Sun 12 May 2024
EGAF00002529182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360960346
Total Bases51.3 Gbp
Sequences flagged as poor quality0
Sequence length1-144
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATTTCAAAGAACTTGGGTCATTTCCCCATCCATGCCAATTCTTGGGTT117613693.258354866492731No Hit
AGGAAATGGTAATATCTCAGGTTTTCCTTATAAAGTTGACATTACCTAGG105427652.9207543479028026No Hit
ATTTATTCTTCTCTCCTGGCCCCCTGCTTCCTCATTAGCATTCCTAAGTG103776112.8750002915832753No Hit
GACTTTTTTGGGTCCTAACAGAGAACATTTGGTATGAGAATGTTTCTTGA97396082.6982487433675053No Hit
ATGAAAAGTCTGTGTAGGCAAATCGGAAGGCCAAAACAAAGGAGCAAAAT91824812.5439029804121476No Hit
TGCTTACTGAACTGTGTTCTTTGTAGAGACAGTTTGTATCTGGGTATAAA91183432.526134269607554No Hit
ATAAAGTCTGGTTGGAAGACAGCCATGCTTATTTGGCTGCTTTCACAGCG82498242.2855208588480243No Hit
ATATTCCAGAAGCAGTGCTCAAGATTTTGAACCAATTAGAGATTTGGGCT79903382.213633183961986No Hit
TTCTCATCACACTTGCTCATCTGTTCATGTGGTGCACACCTGTACAAATT78577562.1769028335317473No Hit
TGTATCACAAATGAATGCTGCTGGTTTATAAAAGAGAGAATGACCAAGTC73007052.022578125520746No Hit
AATTGACTCACTTCTGAACGTTAGGGTCTTCAAAATGATATGTATATACT69077601.913717137228143No Hit
CTGCCTCATATCCATATCAATACCACCACTTCACAGACACACACACACAC66369371.8386886741293185No Hit
AACACAGAGTTTGTCACACTGAGAAAAATAAAAAACAAGAGACAAGCATG54961541.5226475874444114No Hit
TAATTTACTCTCCCCCGTAAGGACCATCAGAAAGAAAAGACTAATGCCTC52864081.4645398195623405No Hit
ATCTCCAAAATTTGTGAATACATGACCTTATATCACAAAAGATACTTTGC52425031.4523764336152316No Hit
AACCAGAGGAAGTAGAAAGAATGAAATAATAAAAATAAAAACAAAATATA50551481.4004718401948784No Hit
AAGTTTCAGGTGATTTCTGAGAATGCTTTTTCTCTTCTGAGTGTACCAAA47918591.3275305869747809No Hit
AACGGGGTGAACATCTAGAAAAAAAGAGCATGTGACCTATAAGGAAAAGA43597041.207806909626577No Hit
ACTACCACCACTGTCAAGATATAGATCATTTCTGCCACTCCAGAGAATAC43134731.1949991315666568No Hit
AGATACTATCACCATGACAGTTTCAACCCACAACAAAACTTATGCACAGA42773571.1849936003773667No Hit
AGACAACCCCATTTTATGAGTTGCTCAGGACCTAAACTCCATCATCACAC33566110.9299112872636708No Hit
TCCAAACAGGAGTTTTGAAGAAGTCTTAGAGGAGTTCAGAACTTCAGTAG31576410.8747888888603848No Hit
CTCTCTCTCTCTCACATCCATCCTATTGTTTTTCAATGTAATTCACAATT30544840.8462104033998238No Hit
AGATACAAAGAACCTTTTGTAGTCCAACCAAATTAGGCAGGGAAGAATGG29788230.8252493751765186No Hit
GAGAAAGAGGGAGTCATCCAGGTATGTGCCTTGGAAGTTTGAGCCACTTG29627590.8207990248324951No Hit
GAAGAGTTTTTTCCACCAAGATCCCAGAAGACAATCTCTTTGTTATCTAT28705970.7952665803351152No Hit
GTAGTGGGGAAATAAAGGAAAATAAGAATGGGAAGAAAGTCCTAATACAT20200030.5596190890176064No Hit
GTGTTGCACACACCAAATATTTATAGCCTATCATGTACCAGAGTGATGGA12247310.33929793495931543No Hit
TGTATCACAAATGAATGCTGCTGAAATCATAGGATTGATTTTGGGGCCTT4441060.1230345673482926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)