FastQCFastQC Report
Fri 23 Dec 2022
EGAF00002529183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360960346
Sequences flagged as poor quality0
Sequence length1-144
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATTTCAAAGAACTTGGGTCATTTCCCCATCCATGCCAATTCTTGGGTT107815452.9869056586066103No Hit
AGGAAATGGTAATATCTCAGGTTTTCCTTATAAAGTTGACATTACCTAGG103066672.8553460551037926No Hit
ATTTATTCTTCTCTCCTGGCCCCCTGCTTCCTCATTAGCATTCCTAAGTG96985572.6868760259887385No Hit
GACTTTTTTGGGTCCTAACAGAGAACATTTGGTATGAGAATGTTTCTTGA95285882.6397880281287187No Hit
ATGAAAAGTCTGTGTAGGCAAATCGGAAGGCCAAAACAAAGGAGCAAAAT91919542.546527368410712No Hit
TGCTTACTGAACTGTGTTCTTTGTAGAGACAGTTTGTATCTGGGTATAAA90002152.4934082371474675No Hit
TTCTCATCACACTTGCTCATCTGTTCATGTGGTGCACACCTGTACAAATT77700432.1526029343954587No Hit
ATAAAGTCTGGTTGGAAGACAGCCATGCTTATTTGGCTGCTTTCACAGCG76618142.12261930843783No Hit
ATATTCCAGAAGCAGTGCTCAAGATTTTGAACCAATTAGAGATTTGGGCT74355832.0599445569015495No Hit
TGTATCACAAATGAATGCTGCTGGTTTATAAAAGAGAGAATGACCAAGTC72249982.0016043535153303No Hit
AATTGACTCACTTCTGAACGTTAGGGTCTTCAAAATGATATGTATATACT64412841.784485213231705No Hit
CTGCCTCATATCCATATCAATACCACCACTTCACAGACACACACACACAC63952591.771734505152541No Hit
TAATTTACTCTCCCCCGTAAGGACCATCAGAAAGAAAAGACTAATGCCTC54731771.5162820682801539No Hit
ATCTCCAAAATTTGTGAATACATGACCTTATATCACAAAAGATACTTTGC54344171.5055440466582444No Hit
AACACAGAGTTTGTCACACTGAGAAAAATAAAAAACAAGAGACAAGCATG52758011.461601269630875No Hit
AACCAGAGGAAGTAGAAAGAATGAAATAATAAAAATAAAAACAAAATATA51528401.4275363089329485No Hit
AAGTTTCAGGTGATTTCTGAGAATGCTTTTTCTCTTCTGAGTGTACCAAA51371361.4231856925358777No Hit
AACGGGGTGAACATCTAGAAAAAAAGAGCATGTGACCTATAAGGAAAAGA43624861.2085776314055285No Hit
AGATACTATCACCATGACAGTTTCAACCCACAACAAAACTTATGCACAGA43432591.2032510075220284No Hit
ACTACCACCACTGTCAAGATATAGATCATTTCTGCCACTCCAGAGAATAC40396041.1191268084611155No Hit
TCCAAACAGGAGTTTTGAAGAAGTCTTAGAGGAGTTCAGAACTTCAGTAG30943710.8572606476834438No Hit
AGATACAAAGAACCTTTTGTAGTCCAACCAAATTAGGCAGGGAAGAATGG28688760.794789796660933No Hit
AGACAACCCCATTTTATGAGTTGCTCAGGACCTAAACTCCATCATCACAC28130630.7793274333796212No Hit
GAGAAAGAGGGAGTCATCCAGGTATGTGCCTTGGAAGTTTGAGCCACTTG27404050.7591983524971466No Hit
GAAGAGTTTTTTCCACCAAGATCCCAGAAGACAATCTCTTTGTTATCTAT26323250.7292560053120073No Hit
GTAGTGGGGAAATAAAGGAAAATAAGAATGGGAAGAAAGTCCTAATACAT19604070.5431086881770664No Hit
GTGTTGCACACACCAAATATTTATAGCCTATCATGTACCAGAGTGATGGA11451770.3172583949152132No Hit
TGTATCACAAATGAATGCTGCTGAAATCATAGGATTGATTTTGGGGCCTT4194050.11619143339362822No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)