FastQCFastQC Report
Wed 27 Sep 2023
EGAF00002529185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54387261
Sequences flagged as poor quality0
Sequence length1-144
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCAGTTGTAATTTTGTGAGCACCTAGTGAATCTAGAAGCAGAGCGAT16884443.104484338713067No Hit
TAGAAGGGTCTATGCTGTGAAGATTGTTGTTTACCAGTTTTATGCACTCT14684832.700049557560915No Hit
GTGATGTTTGGCACAATCGGATTGGTTCCCATGAGGTCCAGGGCTGCGGC14659032.695305799643045No Hit
ATCTTCCAGAGATGCTGTCTGCATTAGCAAGCAAAATGTGCATATTTTTG14094762.59155540118117No Hit
CCGTCTCTTGTATCAAATCAATTTTGGAATGAAGTGAGGATATAAACAAG13958142.5664355482067758No Hit
GGTCTAGAGTGATGGTGTCCTTCTCAAGCGTCTAAGTCCTGTCCAGGCTA13446762.4724098534765337No Hit
AACTTCGGTTGACAATGATCCTGCTCAGTGGTCTCCATCGTTTTTGCCAT13317672.448674515894448No Hit
CTCTGGCTTGATCCTTGTATATGAGCCCTAGCATCCAGTCCAAGTATTTC13022032.394316198420068No Hit
TATGGAAAATGTCAGACCCTTCCACAAAGTTAGAGTAGTGTAATGAACCA12918882.3753503600778867No Hit
ATTGGTACTTGCAACACTTTCTCTGGAATTTGGCCATTTCTCTGCTCTTT12747212.343785983265456No Hit
AGACATTTCAAAATGACCCTGTAGGCAAGGCCCAGACATAAAAGTTACAT12172122.238046148343451No Hit
GTGATGCTTCTGCTGATTACCTGCTCCTCTGTGCACTCATGGTCACTTCT12006232.2075445203978923No Hit
AGTGTGACATGCTCATAGAGACTTCCTCTTGAAGCATACAGAATGGAGAG11899752.1879664063244517No Hit
CTCTGAGAAGCATTCCATTCTGTAAAATTTCCCGGCATGTGCATCTTATG11886292.185491562077377No Hit
TATTCCTACTGTTGCTGCTGTTGTTATTTTCATGGTCATCCCACAGCCTC11260422.070414981920123No Hit
AAGAAGTTGCTGCTTGAAAATCCGGAGGTGGAGTTCAGTAATAAACTGAA11065322.034542610998557No Hit
ATTGTGTGTCATCCAAAAGACAAACAGACTGGCCGAATAGAAAGCAGATC10606261.9501368160459487No Hit
GTACGTTTGCTTTTGTGGTAATTTCTCTGGCCATGCTCTCCCTTCCCTCC9449881.737517173368962No Hit
GCTGAAGCACAATCACTTAGTATATTTCCTTACAGTCTTCTTTCAAATGC9311641.7120994565253067No Hit
CTATGAATGTATTCTCCATCTAAAACTCAAGTGACTCTTAGAAAGGGATC4036560.7421885062386209No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)