FastQCFastQC Report
Mon 13 May 2024
EGAF00002529187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438731754
Total Bases62.5 Gbp
Sequences flagged as poor quality0
Sequence length1-144
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAAGGGTCTATGCTGTGAAGATTGTTGTTTACCAGTTTTATGCACTCT123803542.8218504558026587No Hit
AACTTCGGTTGACAATGATCCTGCTCAGTGGTCTCCATCGTTTTTGCCAT118025722.690156774018231No Hit
CTCTGGCTTGATCCTTGTATATGAGCCCTAGCATCCAGTCCAAGTATTTC113716782.5919432309884733No Hit
AAGAAGTTGCTGCTTGAAAATCCGGAGGTGGAGTTCAGTAATAAACTGAA111373892.538541807940348No Hit
TATGGAAAATGTCAGACCCTTCCACAAAGTTAGAGTAGTGTAATGAACCA110291352.5138675054735153No Hit
GGTCTAGAGTGATGGTGTCCTTCTCAAGCGTCTAAGTCCTGTCCAGGCTA109445132.494579637834922No Hit
CTCCCAGTTGTAATTTTGTGAGCACCTAGTGAATCTAGAAGCAGAGCGAT109017202.4848258418970057No Hit
CTCTGAGAAGCATTCCATTCTGTAAAATTTCCCGGCATGTGCATCTTATG105540582.405583344213558No Hit
CCGTCTCTTGTATCAAATCAATTTTGGAATGAAGTGAGGATATAAACAAG104818652.3891284148992784No Hit
AGACATTTCAAAATGACCCTGTAGGCAAGGCCCAGACATAAAAGTTACAT103143082.3509371970372586No Hit
GTGATGTTTGGCACAATCGGATTGGTTCCCATGAGGTCCAGGGCTGCGGC100532012.2914231551154147No Hit
TATTCCTACTGTTGCTGCTGTTGTTATTTTCATGGTCATCCCACAGCCTC98428642.2434811044016656No Hit
ATTGGTACTTGCAACACTTTCTCTGGAATTTGGCCATTTCTCTGCTCTTT97591372.22439723385055No Hit
AGTGTGACATGCTCATAGAGACTTCCTCTTGAAGCATACAGAATGGAGAG92433572.1068356497396357No Hit
ATCTTCCAGAGATGCTGTCTGCATTAGCAAGCAAAATGTGCATATTTTTG84673481.9299601459893418No Hit
ATTGTGTGTCATCCAAAAGACAAACAGACTGGCCGAATAGAAAGCAGATC82148961.8724188356788054No Hit
GTGATGCTTCTGCTGATTACCTGCTCCTCTGTGCACTCATGGTCACTTCT79545981.813089188889665No Hit
GCTGAAGCACAATCACTTAGTATATTTCCTTACAGTCTTCTTTCAAATGC77017531.7554583022044035No Hit
GTACGTTTGCTTTTGTGGTAATTTCTCTGGCCATGCTCTCCCTTCCCTCC67874511.5470617155283453No Hit
CTATGAATGTATTCTCCATCTAAAACTCAAGTGACTCTTAGAAAGGGATC33789170.7701555607028162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)