FastQCFastQC Report
Wed 15 May 2024
EGAF00002529190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58779209
Total Bases8.3 Gbp
Sequences flagged as poor quality0
Sequence length1-144
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATATCATTCTTGCCTGTGTACATACATCTCTTCTCCACTGCTCAGAAC19677233.3476513778877157No Hit
GATTATGGTCGCTGAAAAATCTTTGTCAGAGGCAGTTGGGATAACCAGCC17683103.0083936651818504No Hit
GTGATGTTTGGCACAATCGGATTGGTTCCCATGAGGTCCAGGGCTGCGGC16088452.7370987588485582No Hit
CTCTGAGAAGCATTCCATTCTGTAAAATTTCCCGGCATGTGCATCTTATG16066992.733447808050632No Hit
CTATAGTTGAAGATGAGATTTAGGTGGGGACACAGCCAAACAATGTAAGA15079942.565522785446126No Hit
GTACGTTTGCTTTTGTGGTAATTTCTCTGGCCATGCTCTCCCTTCCCTCC14679722.497434084218452No Hit
ATTGTGTGTCATCCAAAAGACAAACAGACTGGCCGAATAGAAAGCAGATC14442292.4570405498311487No Hit
GACTGACAGCAGCAATCTAGGAAATGGGACAATGAGTGAGATGAAAACTG14434912.455785003843791No Hit
ACTGCTTCTCAACTAGGAGGATTTTTGTCCCCTCTGGGTACGGGCAATGT14185512.4133550351111395No Hit
GCTGTTTAGCAGTCTTCACTGTTGATGCCTTCAGGTACTGACAAATCTGG14166482.4101174957968556No Hit
AACTTCGGTTGACAATGATCCTGCTCAGTGGTCTCCATCGTTTTTGCCAT13353472.2718015820866184No Hit
ACAGGAAAACCTTACCTAGCCAAACATCTGCATGGTGATACTGTGGATGT12573542.1391135086557562No Hit
AAGAAGTTGCTGCTTGAAAATCCGGAGGTGGAGTTCAGTAATAAACTGAA12221952.0792981409464018No Hit
ATGATGCATGGAGGAACAGGTGGGAAGGTGGCCCAGTCACAGTCCCCACC12126422.063045795665607No Hit
ATTGTTCCAAATGCTTCTTTCTTCCTGCCCTGCAATCCTCTGGGCCATAA11187511.9033107437699612No Hit
TCATACCGCTCTAGGTCCCAGGAAAGTAGGATCAAGTAGGCACTGCACTC10886661.8521276800441462No Hit
CCTCAATGGAGAGAGAAAATTATCTGGAAGGCCTGAGTTCTCAGAGACTG10856651.8470221332852574No Hit
AACATATTGCCACCTATCTGCTGGCTTCTTTCGTTGACATGAGGCAGTGG9863181.6780048877486595No Hit
GCTGTTTAGCAGTCTTCACTGTTGATACCTTCAGGTGCTGGATAATCTGA8185251.3925417063710401No Hit
CCAAAAAGGGAAGATTCTAGCAGCAAGGTCCCATTCTGACTCCAAGATGT7731411.3153307319940288No Hit
TTCTTTCCCTTCTGGTAATCACTTAGGGTTCTATGAGTAGCCACATGTTT7483301.2731202286168908No Hit
GCTGTTTAGCAGTCTTCACTGTTGATACCTCCAGGTACTGGAAAATCCAA1242350.21135874761431375No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)