FastQCFastQC Report
Tue 5 Sep 2023
EGAF00002529191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58779209
Sequences flagged as poor quality0
Sequence length1-144
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATATCATTCTTGCCTGTGTACATACATCTCTTCTCCACTGCTCAGAAC18827303.203054331677039No Hit
GATTATGGTCGCTGAAAAATCTTTGTCAGAGGCAGTTGGGATAACCAGCC15938822.7116424788907927No Hit
CTCTGAGAAGCATTCCATTCTGTAAAATTTCCCGGCATGTGCATCTTATG15150432.5775151210353986No Hit
GTGATGTTTGGCACAATCGGATTGGTTCCCATGAGGTCCAGGGCTGCGGC14080962.3955681336235743No Hit
CTATAGTTGAAGATGAGATTTAGGTGGGGACACAGCCAAACAATGTAAGA13951742.373584169872038No Hit
AACTTCGGTTGACAATGATCCTGCTCAGTGGTCTCCATCGTTTTTGCCAT13147952.2368368380050843No Hit
GACTGACAGCAGCAATCTAGGAAATGGGACAATGAGTGAGATGAAAACTG13090462.227056168789206No Hit
GCTGTTTAGCAGTCTTCACTGTTGATGCCTTCAGGTACTGACAAATCTGG13053762.2208124644889318No Hit
ATTGTGTGTCATCCAAAAGACAAACAGACTGGCCGAATAGAAAGCAGATC12919302.1979370290607347No Hit
GTACGTTTGCTTTTGTGGTAATTTCTCTGGCCATGCTCTCCCTTCCCTCC12320062.095989416938224No Hit
AAGAAGTTGCTGCTTGAAAATCCGGAGGTGGAGTTCAGTAATAAACTGAA12055292.0509445780394904No Hit
ACAGGAAAACCTTACCTAGCCAAACATCTGCATGGTGATACTGTGGATGT11972942.0369345222049517No Hit
ACTGCTTCTCAACTAGGAGGATTTTTGTCCCCTCTGGGTACGGGCAATGT11683091.9876228684873931No Hit
ATGATGCATGGAGGAACAGGTGGGAAGGTGGCCCAGTCACAGTCCCCACC10401441.769578083298127No Hit
CCTCAATGGAGAGAGAAAATTATCTGGAAGGCCTGAGTTCTCAGAGACTG9937211.6905994771042259No Hit
TCATACCGCTCTAGGTCCCAGGAAAGTAGGATCAAGTAGGCACTGCACTC9643321.6406005055290895No Hit
ATTGTTCCAAATGCTTCTTTCTTCCTGCCCTGCAATCCTCTGGGCCATAA9536971.6225073733129005No Hit
AACATATTGCCACCTATCTGCTGGCTTCTTTCGTTGACATGAGGCAGTGG9498171.6159063998292322No Hit
GCTGTTTAGCAGTCTTCACTGTTGATACCTTCAGGTGCTGGATAATCTGA7704011.3106692197916443No Hit
TTCTTTCCCTTCTGGTAATCACTTAGGGTTCTATGAGTAGCCACATGTTT7646821.3009395890305362No Hit
CCAAAAAGGGAAGATTCTAGCAGCAAGGTCCCATTCTGACTCCAAGATGT7178941.2213400149702593No Hit
GCTGTTTAGCAGTCTTCACTGTTGATACCTCCAGGTACTGGAAAATCCAA1129160.19210193862935446No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)