FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002529192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433677
Sequences flagged as poor quality0
Sequence length2-144
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTGGCTTGATCCTTGTATATGAGCCCTAGCATCCAGTCCAAGTATTTC522683.6457305236814146No Hit
CTCCCAGTTGTAATTTTGTGAGCACCTAGTGAATCTAGAAGCAGAGCGAT426082.971938588677924No Hit
CTCTGAGAAGCATTCCATTCTGTAAAATTTCCCGGCATGTGCATCTTATG392412.7370879214774315No Hit
GTGATGTTTGGCACAATCGGATTGGTTCCCATGAGGTCCAGGGCTGCGGC373422.6046313081677392No Hit
ATCTTCCAGAGATGCTGTCTGCATTAGCAAGCAAAATGTGCATATTTTTG367302.5619438688072695No Hit
AGTGTGACATGCTCATAGAGACTTCCTCTTGAAGCATACAGAATGGAGAG361772.523371721803447No Hit
AACTTCGGTTGACAATGATCCTGCTCAGTGGTCTCCATCGTTTTTGCCAT359192.505376036582857No Hit
AAGAAGTTGCTGCTTGAAAATCCGGAGGTGGAGTTCAGTAATAAACTGAA339592.368664629480699No Hit
AGACATTTCAAAATGACCCTGTAGGCAAGGCCCAGACATAAAAGTTACAT318192.219398093154874No Hit
ATTGGTACTTGCAACACTTTCTCTGGAATTTGGCCATTTCTCTGCTCTTT316932.2106095026983064No Hit
ATTGTGTGTCATCCAAAAGACAAACAGACTGGCCGAATAGAAAGCAGATC316382.206773213213297No Hit
GGTCTAGAGTGATGGTGTCCTTCTCAAGCGTCTAAGTCCTGTCCAGGCTA314722.1951945940403594No Hit
TAGAAGGGTCTATGCTGTGAAGATTGTTGTTTACCAGTTTTATGCACTCT312972.182988218406238No Hit
CCGTCTCTTGTATCAAATCAATTTTGGAATGAAGTGAGGATATAAACAAG299512.0891037521003684No Hit
GTGATGCTTCTGCTGATTACCTGCTCCTCTGTGCACTCATGGTCACTTCT289392.018516025576193No Hit
GTACGTTTGCTTTTGTGGTAATTTCTCTGGCCATGCTCTCCCTTCCCTCC280091.9536478579205776No Hit
TATTCCTACTGTTGCTGCTGTTGTTATTTTCATGGTCATCCCACAGCCTC242781.6934079294011133No Hit
TATGGAAAATGTCAGACCCTTCCACAAAGTTAGAGTAGTGTAATGAACCA215871.5057087475072837No Hit
GCTGAAGCACAATCACTTAGTATATTTCCTTACAGTCTTCTTTCAAATGC207381.4464903880023186No Hit
CTATGAATGTATTCTCCATCTAAAACTCAAGTGACTCTTAGAAAGGGATC171971.1995030958856143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)