FastQCFastQC Report
Wed 8 May 2024
EGAF00002529196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3300380
Total Bases449.1 Mbp
Sequences flagged as poor quality0
Sequence length1-144
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAAGTGGAGAAAGGGTGTTTATCTTTCTTCATTCAAACTGTTTATTCT1490914.517388906731952No Hit
CAAAACCACTTTGTTCTCAGATCTGTCACTGTGGTTTTTGTCAGATAAGT520371.5766972288039558No Hit
CTGCTTACAGCTGTTGTTCTGCTGCTAAGTGGCTGTGTAAGCTTAGACAC494141.4972215320660045No Hit
TAGTCTTTCTCTCTGGTTGCTGGCTCAGAGCTCTTAAAACCTCCGAATTT451611.3683575830661925No Hit
ACCATCTGAAACTTGAAAGGATGATGATTTGAACTTAATTTTAGTTAGCC438521.328695483550379No Hit
CTGGTTGCTGAAATCTGTAGGCATTCTTCATTGAAATTTTGTTTATGCTC405631.2290402923299741No Hit
CTGTTGAAACATTGTCTTTTGTGATGTGATTGCCTCGTGCAAAAAAAATG392751.1900144831807247No Hit
ATGTTTTACAATCTCCTCCCAGTGCTATATCACAGTCAGAACCCAAAATA391871.1873481235494094No Hit
AAATCTAAACAGGGCTCCTACGTCCAGCAACTCCTATTTCTATATTCCAT388771.1779552657572765No Hit
ATCTCCTCACTCAGGTGTCTGATGGCCCTGAAGACACAGTTGTATAGGAT385061.1667141359479818No Hit
TATTGCATACCTTTTCTTGCTAAGTATAAATATTCCATTGTATGAACATA375261.1370205855083355No Hit
AGGAATGTATATGACTGCCAAGTACAATTACTTTTGGTCATTCGAGTTTT369811.12050733551894No Hit
GGTGTTAAAACAACAGTGGTAATCCTGGAACCCTCAAGTGACTTTGTCAT358041.0848447754500996No Hit
TCTAGTTGCTGGAAAATGTGTCTCCTTATAGAAAGCAAAAATGTGCCTCT353041.069695004817627No Hit
ATCCTCTGCATACCTTCTTTTTGTGAATAATATCACTTTTCCCACTTACC348681.0564844048261108No Hit
CCTCTTCACCACCAACTAGTACCTCTAAATCCTTTGCAATTTCCAGAACA341231.0339112465837268No Hit
ATTTCCTCTAAAATGGGTCATTTCTGAAATAGACATATGTGTTTATAAAT329910.9996121658718087No Hit
CTTAAGTGGAGAAAGGGTGTAGGCACAAGAAACAAGTCAGAAAGACCTTG317830.9630103200237548No Hit
AAAGTTGCATGGTGCTGGTGGGAGTGTCACTTAGCATACTTATGAATTAT307840.9327410783000746No Hit
TTTCTTCAGAAGAGGAGGATAATATGGTTTAGAGATTTAAAAGCCAGGTT285080.8637793223810591No Hit
ACAACTGTGAACAATTGGAAAAAGCTGCAAAACAATGTGGACATTCTTTT283430.8587798980723432No Hit
GTTGATGGTTCTCGTCAATACTGATGATTAACCAGGATTAACGTTTTCTC279900.8480841600058174No Hit
TATGCTGGTGTCAAAAATTCAAATGCTATCTAATCTGTCACAGGAGTGGA275020.8332979838685242No Hit
TTGATCCTTTCTAATTTGCAATACATTGTCCCTGTGTTTGAGTGAGAGAT256980.7786376114265631No Hit
GGAAAGCCAGAAACTGATATTGCCCAAAGAACCTTAGGTTTCAGGGCCAT256260.776456044455487No Hit
TGTGGATGATAGAGTCAGGAACCTCACAGAATTATTCCCAGTGAATAGGA252350.7646089238208933No Hit
TCCAGTTCATTGGCATATAGTTGTTCATAATAGTCTTTGATGATGTTTGA236070.7152812706415624No Hit
ATGACTACATCAGGTGGTGATCTATGGAGACACTATCTTTTGTTTTTACT230720.6990710160648168No Hit
TAAATGAATGCTGGAAGAAAGAGCTTCCTGAGTGGGAAAATAAGAAGGGG164450.49827595610202463No Hit
GAGTCAGCCTCCTTTTTAAGTTTTACAGTGGGAGGTAGACTCTTACTTCT156610.4745211157503076No Hit
CTTTCAAATTTTCCACAGTGTTTCATATGTATTTATTGTGTAAAATACAT137150.41555820844872404No Hit
CG111510.33787018464540447No Hit
CTTTCAAATTTTCCACAGTGTTTCATATATATTTATTGTGTAAAATACAT111020.33638550712342213No Hit
GAGTATCTTTCTGGGGAAAAAAAGTTTTGTTATAATTTTTTTTTTATTTT101400.3072373484265448No Hit
G80160.24288112277980112No Hit
TCTAGTTGCTGGAAAATGTGCAGCGAGGCAGCTTGGAAGCAAAGCTCGGG74480.22567098334131225No Hit
CTGGTTGCTGAAATCTGTGATGATTCTTAATCCAGGAAGACTTCTAACAC71040.21524794114617102No Hit
AAAGTTGCATGGTGCTGGTGCATCGGAGGAATAATATCGGACACACGTTT67960.20591568243656788No Hit
GG60360.1828880310752095No Hit
CT59250.1795247819948006No Hit
C55480.16810185493791624No Hit
GC50540.15313388155303329No Hit
CAAAACCACTTTGTTCTCAGGCCTGCCACCCAGGAGTCAACCTTAGCCCC47510.1439531205497549No Hit
AAAGTTGCATGGTGCTGGTACCAAAACAGAGATATAGATCAATGGAACAG46600.14119586229464487No Hit
AAAGTTGCATGGTGCTGGCACTTTGCTAAGACTACAGTGTCTACTTTATA46380.14052927238681606No Hit
AAAGTTGCATGGTGCTGGTGGGAGGGTAGCAGTGAGGACGACCAGAGGTC45440.1376811155079112No Hit
AAAGTTGCATGGTGCTGGTGTGCGGGCTGGTGTGGGTGCCCATCATCAAA40230.12189505450887472No Hit
TGTGGATGATAGAGTCAGGAAGAAGGACTTGAGGCTGCGTAAGAAATTCG40010.12122846460104594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)