FastQCFastQC Report
Thu 9 May 2024
EGAF00002529197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3300380
Total Bases449.2 Mbp
Sequences flagged as poor quality0
Sequence length1-144
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAAGTGGAGAAAGGGTGTTTATCTTTCTTCATTCAAACTGTTTATTCT1250873.790078718208206No Hit
CAAAACCACTTTGTTCTCAGATCTGTCACTGTGGTTTTTGTCAGATAAGT476791.4446518279713245No Hit
CTGCTTACAGCTGTTGTTCTGCTGCTAAGTGGCTGTGTAAGCTTAGACAC424511.2862458262381908No Hit
TAGTCTTTCTCTCTGGTTGCTGGCTCAGAGCTCTTAAAACCTCCGAATTT414291.2552796950654166No Hit
ACCATCTGAAACTTGAAAGGATGATGATTTGAACTTAATTTTAGTTAGCC379291.1492313006381083No Hit
CTGGTTGCTGAAATCTGTAGGCATTCTTCATTGAAATTTTGTTTATGCTC362561.098540168101855No Hit
AAATCTAAACAGGGCTCCTACGTCCAGCAACTCCTATTTCTATATTCCAT348291.0553027227167782No Hit
CTGTTGAAACATTGTCTTTTGTGATGTGATTGCCTCGTGCAAAAAAAATG343371.040395348414425No Hit
ATGTTTTACAATCTCCTCCCAGTGCTATATCACAGTCAGAACCCAAAATA337061.0212763378762444No Hit
ATCTCCTCACTCAGGTGTCTGATGGCCCTGAAGACACAGTTGTATAGGAT324880.9843714966155412No Hit
TATTGCATACCTTTTCTTGCTAAGTATAAATATTCCATTGTATGAACATA321010.9726455741460074No Hit
AGGAATGTATATGACTGCCAAGTACAATTACTTTTGGTCATTCGAGTTTT319220.9672219562595822No Hit
TCTAGTTGCTGGAAAATGTGTCTCCTTATAGAAAGCAAAAATGTGCCTCT317320.9614650434192425No Hit
GGTGTTAAAACAACAGTGGTAATCCTGGAACCCTCAAGTGACTTTGTCAT301850.9145916530823724No Hit
CCTCTTCACCACCAACTAGTACCTCTAAATCCTTTGCAATTTCCAGAACA294610.8926547852065521No Hit
AAAGTTGCATGGTGCTGGTGGGAGTGTCACTTAGCATACTTATGAATTAT288760.8749295535665591No Hit
GTTGATGGTTCTCGTCAATACTGATGATTAACCAGGATTAACGTTTTCTC285130.8639308200873839No Hit
CTTAAGTGGAGAAAGGGTGTAGGCACAAGAAACAAGTCAGAAAGACCTTG283410.8587192989898133No Hit
NN281880.8540834691762768No Hit
ATTTCCTCTAAAATGGGTCATTTCTGAAATAGACATATGTGTTTATAAAT277020.8393578921215132No Hit
ACAACTGTGAACAATTGGAAAAAGCTGCAAAACAATGTGGACATTCTTTT251380.7616698683181936No Hit
ATCCTCTGCATACCTTCTTTTTGTGAATAATATCACTTTTCCCACTTACC250930.7603063889612711No Hit
TTTCTTCAGAAGAGGAGGATAATATGGTTTAGAGATTTAAAAGCCAGGTT246030.745459613741448No Hit
TATGCTGGTGTCAAAAATTCAAATGCTATCTAATCTGTCACAGGAGTGGA238390.7223107642150297No Hit
TTGATCCTTTCTAATTTGCAATACATTGTCCCTGTGTTTGAGTGAGAGAT217220.6581666353571407No Hit
TGTGGATGATAGAGTCAGGAACCTCACAGAATTATTCCCAGTGAATAGGA212150.6428047679358134No Hit
GGAAAGCCAGAAACTGATATTGCCCAAAGAACCTTAGGTTTCAGGGCCAT202980.6150200885958587No Hit
TCCAGTTCATTGGCATATAGTTGTTCATAATAGTCTTTGATGATGTTTGA198140.6003551106236251No Hit
ATGACTACATCAGGTGGTGATCTATGGAGACACTATCTTTTGTTTTTACT187170.5671165138559803No Hit
TAAATGAATGCTGGAAGAAAGAGCTTCCTGAGTGGGAAAATAAGAAGGGG152980.46352238227113246No Hit
GAGTCAGCCTCCTTTTTAAGTTTTACAGTGGGAGGTAGACTCTTACTTCT142010.4302837855034875No Hit
N136390.41325544331258823No Hit
CTTTCAAATTTTCCACAGTGTTTCATATGTATTTATTGTGTAAAATACAT111860.3389306685896776No Hit
CTTTCAAATTTTCCACAGTGTTTCATATATATTTATTGTGTAAAATACAT97500.29542052733321617No Hit
GAGTATCTTTCTGGGGAAAAAAAGTTTTGTTATAATTTTTTTTTTATTTT90730.27490773789684825No Hit
AAAGTTGCATGGTGCTGGTGCATCGGAGGAATAATATCGGACACACGTTT68830.20855174252661815No Hit
CTGGTTGCTGAAATCTGTGATGATTCTTAATCCAGGAAGACTTCTAACAC66730.20218883886097966No Hit
TCTAGTTGCTGGAAAATGTGCAGCGAGGCAGCTTGGAAGCAAAGCTCGGG64430.19521994437004223No Hit
AAAGTTGCATGGTGCTGGCACTTTGCTAAGACTACAGTGTCTACTTTATA44060.13349977881334876No Hit
CAAAACCACTTTGTTCTCAGGCCTGCCACCCAGGAGTCAACCTTAGCCCC41440.1255612990019331No Hit
AAAGTTGCATGGTGCTGGTACCAAAACAGAGATATAGATCAATGGAACAG39820.12065277331701199No Hit
AAAGTTGCATGGTGCTGGTGGGAGGGTAGCAGTGAGGACGACCAGAGGTC38710.11728952423660306No Hit
TGTGGATGATAGAGTCAGGAAGAAGGACTTGAGGCTGCGTAAGAAATTCG38230.11583514625588569No Hit
AAAGTTGCATGGTGCTGGTGTGCGGGCTGGTGTGGGTGCCCATCATCAAA35840.10859355589356377No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)