FastQCFastQC Report
Mon 13 May 2024
EGAF00002529199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331567793
Total Bases47.1 Gbp
Sequences flagged as poor quality0
Sequence length1-144
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAAGTGGAGAAAGGGTGTTTATCTTTCTTCATTCAAACTGTTTATTCT80626962.4316885325469473No Hit
CAAAACCACTTTGTTCTCAGATCTGTCACTGTGGTTTTTGTCAGATAAGT67581622.0382444081352618No Hit
TCTAGTTGCTGGAAAATGTGTCTCCTTATAGAAAGCAAAAATGTGCCTCT67113842.0241362827420333No Hit
CTGGTTGCTGAAATCTGTAGGCATTCTTCATTGAAATTTTGTTTATGCTC66416222.003096241618377No Hit
TAGTCTTTCTCTCTGGTTGCTGGCTCAGAGCTCTTAAAACCTCCGAATTT64105051.933391944373801No Hit
CTGTTGAAACATTGTCTTTTGTGATGTGATTGCCTCGTGCAAAAAAAATG60636921.8287940288579236No Hit
CTGCTTACAGCTGTTGTTCTGCTGCTAAGTGGCTGTGTAAGCTTAGACAC60412071.8220126102537346No Hit
ACCATCTGAAACTTGAAAGGATGATGATTTGAACTTAATTTTAGTTAGCC59827121.8043706675696332No Hit
ATGTTTTACAATCTCCTCCCAGTGCTATATCACAGTCAGAACCCAAAATA56529541.7049164965187074No Hit
TATTGCATACCTTTTCTTGCTAAGTATAAATATTCCATTGTATGAACATA52486401.5829764261814174No Hit
CCTCTTCACCACCAACTAGTACCTCTAAATCCTTTGCAATTTCCAGAACA51053171.5397505752315335No Hit
ATCTCCTCACTCAGGTGTCTGATGGCCCTGAAGACACAGTTGTATAGGAT50373181.5192422504075962No Hit
TTTCTTCAGAAGAGGAGGATAATATGGTTTAGAGATTTAAAAGCCAGGTT49465641.4918710756686793No Hit
ATTTCCTCTAAAATGGGTCATTTCTGAAATAGACATATGTGTTTATAAAT46488751.4020888331575678No Hit
ATCCTCTGCATACCTTCTTTTTGTGAATAATATCACTTTTCCCACTTACC46046371.3887467652806678No Hit
AGGAATGTATATGACTGCCAAGTACAATTACTTTTGGTCATTCGAGTTTT42483061.2812782452606908No Hit
AAATCTAAACAGGGCTCCTACGTCCAGCAACTCCTATTTCTATATTCCAT41264391.2445234691416485No Hit
TATGCTGGTGTCAAAAATTCAAATGCTATCTAATCTGTCACAGGAGTGGA40497041.221380389017458No Hit
TGTGGATGATAGAGTCAGGAACCTCACAGAATTATTCCCAGTGAATAGGA40104311.2095357524667663No Hit
TTGATCCTTTCTAATTTGCAATACATTGTCCCTGTGTTTGAGTGAGAGAT38309751.1554122809509426No Hit
GGTGTTAAAACAACAGTGGTAATCCTGGAACCCTCAAGTGACTTTGTCAT38243421.1534117850825154No Hit
ACAACTGTGAACAATTGGAAAAAGCTGCAAAACAATGTGGACATTCTTTT33557211.0120768876969906No Hit
TCCAGTTCATTGGCATATAGTTGTTCATAATAGTCTTTGATGATGTTTGA32708540.9864812171307604No Hit
TAAATGAATGCTGGAAGAAAGAGCTTCCTGAGTGGGAAAATAAGAAGGGG31378800.9463765981637426No Hit
GTTGATGGTTCTCGTCAATACTGATGATTAACCAGGATTAACGTTTTCTC31317400.9445247898368706No Hit
AAAGTTGCATGGTGCTGGTGGGAGTGTCACTTAGCATACTTATGAATTAT31241950.942249237096439No Hit
ATGACTACATCAGGTGGTGATCTATGGAGACACTATCTTTTGTTTTTACT31083800.9374794734662302No Hit
CTTAAGTGGAGAAAGGGTGTAGGCACAAGAAACAAGTCAGAAAGACCTTG29210410.8809785092727628No Hit
CTTTCAAATTTTCCACAGTGTTTCATATGTATTTATTGTGTAAAATACAT22818490.688199833691326No Hit
GAGTCAGCCTCCTTTTTAAGTTTTACAGTGGGAGGTAGACTCTTACTTCT21660770.6532832940140239No Hit
GGAAAGCCAGAAACTGATATTGCCCAAAGAACCTTAGGTTTCAGGGCCAT20583940.6208063760885243No Hit
CTTTCAAATTTTCCACAGTGTTTCATATATATTTATTGTGTAAAATACAT20507350.6184964412390922No Hit
GAGTATCTTTCTGGGGAAAAAAAGTTTTGTTATAATTTTTTTTTTATTTT16127930.48641425194153276No Hit
CTGGTTGCTGAAATCTGTGATGATTCTTAATCCAGGAAGACTTCTAACAC7379180.2225541851708136No Hit
AAAGTTGCATGGTGCTGGCACTTTGCTAAGACTACAGTGTCTACTTTATA5009630.15108916202847242No Hit
AAAGTTGCATGGTGCTGGTACCAAAACAGAGATATAGATCAATGGAACAG4155940.12534208954366083No Hit
AAAGTTGCATGGTGCTGGTGGGAGGGTAGCAGTGAGGACGACCAGAGGTC3773110.11379603446586865No Hit
CAAAACCACTTTGTTCTCAGGCCTGCCACCCAGGAGTCAACCTTAGCCCC3530180.10646932767682897No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)