FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002529320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17207044
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1885651.0958593469046747TruSeq Adapter, Index 3 (100% over 50bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA275250.1599635591098622No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG214080.12441416433874405No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT200010.11623728049977673No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA193430.11241326517210046No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)