FastQCFastQC Report
Wed 14 Sep 2022
EGAF00002529322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17436431
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT3363441.9289727353034574TruSeq Adapter, Index 18 (97% over 40bp)
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA704970.4043086569722898No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT327440.18779072391592064No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT301900.17314323097427448No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC221640.12711316897362768No Hit
GATCGGAAAAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT220080.12621849046975267TruSeq Adapter, Index 18 (97% over 40bp)
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC214360.12293800262221093No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA178440.10233745655862717No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)