FastQCFastQC Report
Sat 1 Apr 2023
EGAF00002529330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15127182
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT1069720.7071508758207576TruSeq Adapter, Index 21 (97% over 40bp)
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA281260.18593020167272398No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT211380.1397352130753765No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT188680.12472911345946655No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG165400.10933959808244523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)