FastQCFastQC Report
Sun 14 May 2023
EGAF00002529498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26447409
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC4415381.6694943538703544TruSeq Adapter, Index 6 (100% over 50bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT993380.37560579185658605No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA957390.36199765353195845No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG935300.3536452285363757No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA921840.3485558831112719No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT741060.2802013611238817No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA709690.2683400857906345No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC707630.26756118151309266No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG656830.24835325078536047No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC646350.24439066980058424No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG624240.23603068262754964No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA579160.2189855346510503No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG571090.21593419604922356No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA568680.21502295366627408No Hit
CGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCA553570.20930972860139155No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA540160.20423928861991736No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT529610.2002502400140596No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA501260.1895308534760437No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC496780.1878369257268264No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC485160.18344330062729394No Hit
CCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTT477610.18058857863921565No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG471180.17815733858844168No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA462040.1747014234929403No Hit
CCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCG461150.17436490659633236No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG459880.17388470832813907No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT456640.17265963558093725No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC454800.17196391525536586No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC435680.16473447361138477No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG431830.16327875445190113No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC430040.1626019395699594No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC416970.15766005660516688No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA413850.15648035692267626No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT409940.15500195123083702No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA405240.15322483952964921No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC403490.1525631490026112No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC387090.14636216349208347No Hit
GTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA385090.14560594574689717No Hit
CGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCC384900.14553410506110448No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT368440.13931043301822116No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC358310.13548019013885254No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT355070.13425511739165072No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG350420.13249691113409257No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT345760.1307349237878085No Hit
GCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAG327670.12389493428259833No Hit
TGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCC324700.12277195093099667No Hit
CCGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCC322470.12192876814511396No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT320400.12114608277884611No Hit
CTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGG316540.11968658253063656No Hit
GTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA316160.11954290115905115No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT312560.1181817092177158No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC309600.11706250695484006No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT308660.11670708461460251No Hit
GTTGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCAC306180.11576937461057149No Hit
CCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGAC297440.11246470306410733No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG294190.1112358492281796No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA293380.11092958104137914No Hit
CGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGG288800.1091978424049025No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA284220.10746610376842587No Hit
GGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCC283000.10700481094386222No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG270630.10232760418988493No Hit
CGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGGGCG267170.10101934749071262No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC265890.10053536813379337No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)