FastQCFastQC Report
Fri 15 Sep 2023
EGAF00002529558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35414940
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC18719615.285794639211587TruSeq Adapter, Index 11 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN441350.12462254630390451No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA354750.10016958944445478No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)