FastQCFastQC Report
Tue 5 Sep 2023
EGAF00002529570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38036733
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA3501520.9205627623171527No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT2958300.777748183578227No Hit
CGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCA2147940.5647014952624875No Hit
GCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAG2045170.5376828761818214No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA1992970.5239593000797413No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC1968170.5174392869124695No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1933440.5083086394407217No Hit
CCGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCC1902030.5000508324413666No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1758880.4624161596633444No Hit
CCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCG1742720.4581676349543479No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC1506580.3960855418366241No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT1263420.33215786434655153No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT1233720.3243496227712301No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1180010.3102290619964654No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA1165280.30635648965961404No Hit
CACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCG1160080.3049893901245409No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC1097930.2886499216428498No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC1079970.2839281701717127No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT1058150.27819161019954053No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA1018960.2678884119727107No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1017830.267591330727589No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1013310.2664030057471024No Hit
CCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCC970520.25515335399599115No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC946280.24878056693249653No Hit
CCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAAT937480.24646701387314204No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG908390.23881914358943496No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG881680.2317969842467806No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA879070.2311108054416766No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC875020.23004604522686004No Hit
CGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGG852040.2240045168968639No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA849490.22333411231716457No Hit
CTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGG794690.20892698644754795No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG789000.20743106407166986No Hit
CCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTC770650.20260678013540226No Hit
CCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTT759450.19966225805986018No Hit
ACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGGGC752190.19775357678589273No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG748190.19670196175891344No Hit
CGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCC741830.19502989386601632No Hit
CGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGGGCG729260.1917251936437338No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA724330.1904290781229818No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT705430.18546019712050454No Hit
GGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCC699480.1838959197678728No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT688840.18109862379610783No Hit
CCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTT669050.17589575845012767No Hit
GCCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCG660270.1735874634659081No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC644070.16932842260664183No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG641620.168684308402617No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG640260.16832675929344404No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT633960.16667046562595164No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT628530.1652428982268272No Hit
ACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGC624120.1640834926595825No Hit
CGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCC621660.16343674941799025No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC619320.16282155462720732No Hit
CTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAA617720.16240090861641562No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG606760.15951948344249228No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC570900.15009175472562275No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT569660.14976575406725914No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG541330.14231769063867813No Hit
TCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTG540920.14220990009841278No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC536600.1410741558692751No Hit
GCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCA536380.14101631704279124No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA535980.1409111555400933No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT534780.14059567103199952No Hit
AACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT522850.1374592292140337No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG513340.13495901448739037No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG512680.1347854980079388No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC509520.13395472213662515No Hit
GCCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGC508440.13367078607934071No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC502020.13198294396103893No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC500710.13163854003970318No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490080.12884387310550569No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT487480.12816032333796912No Hit
CGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCA485720.1276976127260982No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC482770.12692204664370096No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC481740.1266512557742538No Hit
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT471380.12392757285437736No Hit
GGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTT466430.12262619925849047No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA465500.12238169876471777No Hit
GACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGGG462260.12152989059286454No Hit
GGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCC461960.1214510194658411No Hit
GCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACT461580.12135111603827804No Hit
GGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAG444540.11687123602334618No Hit
CCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCA441270.11601154073879057No Hit
CCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGC438290.11522808754369099No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC431440.11342719680998892No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG431360.11340616450944932No Hit
GTTGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCAC420670.1105957233498471No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA417440.1097465442155613No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC416300.10944683393287222No Hit
CCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAG403730.10614213371058971No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC402660.10586082669087275TruSeq Adapter, Index 8 (100% over 50bp)
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC400500.10529295457630392No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG398030.1046435822971442No Hit
GTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA396470.10423345243662226No Hit
GCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTC391930.10303986938100074No Hit
CCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGG390000.10253246513048321No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA387700.1019277864899701No Hit
GACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAG386720.10167014080836018No Hit
CGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACC381530.10030567031085451No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)