FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002529636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15878633
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC2132421.3429493584239902TruSeq Adapter, Index 9 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN271630.17106636320645485No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA229650.14462831907507404No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT165080.10396360946184725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)