FastQCFastQC Report
Wed 25 Jan 2023
EGAF00002530493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002530493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416330513
Sequences flagged as poor quality0
Sequence length1-144
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATCACAAATGAATGCTGCTGGTTTATAAAAGAGAGAATGACCAAGTC103986052.4976802504984783No Hit
ATATTCCAGAAGCAGTGCTCAAGATTTTGAACCAATTAGAGATTTGGGCT103378782.483094002768901No Hit
GACTTTTTTGGGTCCTAACAGAGAACATTTGGTATGAGAATGTTTCTTGA99781812.3966970203790945No Hit
AATTGACTCACTTCTGAACGTTAGGGTCTTCAAAATGATATGTATATACT96340922.3140489825207693No Hit
AGGAAATGGTAATATCTCAGGTTTTCCTTATAAAGTTGACATTACCTAGG93375252.242815433515919No Hit
ATTTATTCTTCTCTCCTGGCCCCCTGCTTCCTCATTAGCATTCCTAAGTG87007122.0898569113525434No Hit
AACACAGAGTTTGTCACACTGAGAAAAATAAAAAACAAGAGACAAGCATG86247652.0716149142784546No Hit
AGATTTCAAAGAACTTGGGTCATTTCCCCATCCATGCCAATTCTTGGGTT86089602.0678186515721464No Hit
ATAAAGTCTGGTTGGAAGACAGCCATGCTTATTTGGCTGCTTTCACAGCG80881321.942719005080466No Hit
ATGAAAAGTCTGTGTAGGCAAATCGGAAGGCCAAAACAAAGGAGCAAAAT75897221.8230040227678435No Hit
ACTACCACCACTGTCAAGATATAGATCATTTCTGCCACTCCAGAGAATAC75363591.8101865620404334No Hit
AACCAGAGGAAGTAGAAAGAATGAAATAATAAAAATAAAAACAAAATATA74143241.7808745139946058No Hit
ATCTCCAAAATTTGTGAATACATGACCTTATATCACAAAAGATACTTTGC72748011.7473619571093026No Hit
TTCTCATCACACTTGCTCATCTGTTCATGTGGTGCACACCTGTACAAATT69902861.6790232235512366No Hit
AAGTTTCAGGTGATTTCTGAGAATGCTTTTTCTCTTCTGAGTGTACCAAA67828491.629198146233399No Hit
AACGGGGTGAACATCTAGAAAAAAAGAGCATGTGACCTATAAGGAAAAGA67661581.6251890718372592No Hit
TGCTTACTGAACTGTGTTCTTTGTAGAGACAGTTTGTATCTGGGTATAAA66514661.5976407667242014No Hit
AGATACTATCACCATGACAGTTTCAACCCACAACAAAACTTATGCACAGA61506831.4773558045696256No Hit
GAAGAGTTTTTTCCACCAAGATCCCAGAAGACAATCTCTTTGTTATCTAT55998521.3450496240711522No Hit
CTGCCTCATATCCATATCAATACCACCACTTCACAGACACACACACACAC48366771.1617397353722185No Hit
AGATACAAAGAACCTTTTGTAGTCCAACCAAATTAGGCAGGGAAGAATGG47311001.136380796571593No Hit
AGACAACCCCATTTTATGAGTTGCTCAGGACCTAAACTCCATCATCACAC45579991.0948030129129642No Hit
TAATTTACTCTCCCCCGTAAGGACCATCAGAAAGAAAAGACTAATGCCTC45473141.0922365423645997No Hit
TCCAAACAGGAGTTTTGAAGAAGTCTTAGAGGAGTTCAGAACTTCAGTAG45365401.0896486945697397No Hit
GAGAAAGAGGGAGTCATCCAGGTATGTGCCTTGGAAGTTTGAGCCACTTG44231211.0624061561397014No Hit
CTCTCTCTCTCTCACATCCATCCTATTGTTTTTCAATGTAATTCACAATT44170831.060955866090939No Hit
GTAGTGGGGAAATAAAGGAAAATAAGAATGGGAAGAAAGTCCTAATACAT32995630.7925345121172994No Hit
GTGTTGCACACACCAAATATTTATAGCCTATCATGTACCAGAGTGATGGA31411540.7544856554868944No Hit
GCTCAAAAATGGTTGTGTCGTTGATGCCGCCTGCGTGCTGCAGTACCCCT6301050.15134730228144486No Hit
TGTATCACAAATGAATGCTGCTGAAATCATAGGATTGATTTTGGGGCCTT5707020.13707907111771075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)