FastQCFastQC Report
Fri 10 May 2024
EGAF00002530493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002530493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416330513
Total Bases59.1 Gbp
Sequences flagged as poor quality0
Sequence length1-144
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATCACAAATGAATGCTGCTGGTTTATAAAAGAGAGAATGACCAAGTC103986062.497680490692259No Hit
ATATTCCAGAAGCAGTGCTCAAGATTTTGAACCAATTAGAGATTTGGGCT103378792.483094242962682No Hit
GACTTTTTTGGGTCCTAACAGAGAACATTTGGTATGAGAATGTTTCTTGA99781832.3966975007666567No Hit
AATTGACTCACTTCTGAACGTTAGGGTCTTCAAAATGATATGTATATACT96340932.31404922271455No Hit
AGGAAATGGTAATATCTCAGGTTTTCCTTATAAAGTTGACATTACCTAGG93375282.2428161540972615No Hit
ATTTATTCTTCTCTCCTGGCCCCCTGCTTCCTCATTAGCATTCCTAAGTG87007122.0898569113525434No Hit
AACACAGAGTTTGTCACACTGAGAAAAATAAAAAACAAGAGACAAGCATG86247652.0716149142784546No Hit
AGATTTCAAAGAACTTGGGTCATTTCCCCATCCATGCCAATTCTTGGGTT86089632.067819372153489No Hit
ATAAAGTCTGGTTGGAAGACAGCCATGCTTATTTGGCTGCTTTCACAGCG80881351.9427197256618085No Hit
ATGAAAAGTCTGTGTAGGCAAATCGGAAGGCCAAAACAAAGGAGCAAAAT75897231.8230042629616243No Hit
ACTACCACCACTGTCAAGATATAGATCATTTCTGCCACTCCAGAGAATAC75363621.810187282621776No Hit
AACCAGAGGAAGTAGAAAGAATGAAATAATAAAAATAAAAACAAAATATA74143261.7808749943821676No Hit
ATCTCCAAAATTTGTGAATACATGACCTTATATCACAAAAGATACTTTGC72748051.7473629178844259No Hit
TTCTCATCACACTTGCTCATCTGTTCATGTGGTGCACACCTGTACAAATT69902881.6790237039387983No Hit
AAGTTTCAGGTGATTTCTGAGAATGCTTTTTCTCTTCTGAGTGTACCAAA67828501.62919838642718No Hit
AACGGGGTGAACATCTAGAAAAAAAGAGCATGTGACCTATAAGGAAAAGA67661601.625189552224821No Hit
TGCTTACTGAACTGTGTTCTTTGTAGAGACAGTTTGTATCTGGGTATAAA66514671.597641006917982No Hit
AGATACTATCACCATGACAGTTTCAACCCACAACAAAACTTATGCACAGA61506841.4773560447634064No Hit
GAAGAGTTTTTTCCACCAAGATCCCAGAAGACAATCTCTTTGTTATCTAT55998541.345050104458714No Hit
CTGCCTCATATCCATATCAATACCACCACTTCACAGACACACACACACAC48366791.1617402157597803No Hit
AGATACAAAGAACCTTTTGTAGTCCAACCAAATTAGGCAGGGAAGAATGG47311021.1363812769591548No Hit
AGACAACCCCATTTTATGAGTTGCTCAGGACCTAAACTCCATCATCACAC45580041.0948042138818683No Hit
TAATTTACTCTCCCCCGTAAGGACCATCAGAAAGAAAAGACTAATGCCTC45473171.0922372629459423No Hit
TCCAAACAGGAGTTTTGAAGAAGTCTTAGAGGAGTTCAGAACTTCAGTAG45365411.0896489347635203No Hit
GAGAAAGAGGGAGTCATCCAGGTATGTGCCTTGGAAGTTTGAGCCACTTG44231211.0624061561397014No Hit
CTCTCTCTCTCTCACATCCATCCTATTGTTTTTCAATGTAATTCACAATT44170831.060955866090939No Hit
GTAGTGGGGAAATAAAGGAAAATAAGAATGGGAAGAAAGTCCTAATACAT32995630.7925345121172994No Hit
GTGTTGCACACACCAAATATTTATAGCCTATCATGTACCAGAGTGATGGA31411550.7544858956806753No Hit
GCTCAAAAATGGTTGTGTCGTTGATGCCGCCTGCGTGCTGCAGTACCCCT6301050.15134730228144486No Hit
TGTATCACAAATGAATGCTGCTGAAATCATAGGATTGATTTTGGGGCCTT5707020.13707907111771075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)