FastQCFastQC Report
Sat 21 Jan 2023
EGAF00002530494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002530494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416330513
Sequences flagged as poor quality0
Sequence length1-144
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATCACAAATGAATGCTGCTGGTTTATAAAAGAGAGAATGACCAAGTC99873622.3989022394810635No Hit
ATATTCCAGAAGCAGTGCTCAAGATTTTGAACCAATTAGAGATTTGGGCT96009272.306082955778934No Hit
GACTTTTTTGGGTCCTAACAGAGAACATTTGGTATGAGAATGTTTCTTGA95259622.2880768289976383No Hit
AATTGACTCACTTCTGAACGTTAGGGTCTTCAAAATGATATGTATATACT90655672.1774928132639655No Hit
AGGAAATGGTAATATCTCAGGTTTTCCTTATAAAGTTGACATTACCTAGG89604082.152234275463735No Hit
AACACAGAGTTTGTCACACTGAGAAAAATAAAAAACAAGAGACAAGCATG82334291.9776184408563875No Hit
ATTTATTCTTCTCTCCTGGCCCCCTGCTTCCTCATTAGCATTCCTAAGTG81000501.9455816345606165No Hit
AGATTTCAAAGAACTTGGGTCATTTCCCCATCCATGCCAATTCTTGGGTT77682741.8658911027258769No Hit
ATAAAGTCTGGTTGGAAGACAGCCATGCTTATTTGGCTGCTTTCACAGCG74895731.798948855809663No Hit
AACCAGAGGAAGTAGAAAGAATGAAATAATAAAAATAAAAACAAAATATA73676761.7696699545055923No Hit
ATGAAAAGTCTGTGTAGGCAAATCGGAAGGCCAAAACAAAGGAGCAAAAT72721111.7467158358388208No Hit
ATCTCCAAAATTTGTGAATACATGACCTTATATCACAAAAGATACTTTGC70742251.6991848493218658No Hit
ACTACCACCACTGTCAAGATATAGATCATTTCTGCCACTCCAGAGAATAC70464521.6925139474463644No Hit
AAGTTTCAGGTGATTTCTGAGAATGCTTTTTCTCTTCTGAGTGTACCAAA68860151.653977977828399No Hit
TTCTCATCACACTTGCTCATCTGTTCATGTGGTGCACACCTGTACAAATT67969641.6325884814500733No Hit
AACGGGGTGAACATCTAGAAAAAAAGAGCATGTGACCTATAAGGAAAAGA67837151.6294061540476135No Hit
TGCTTACTGAACTGTGTTCTTTGTAGAGACAGTTTGTATCTGGGTATAAA63691031.5298189301825205No Hit
AGATACTATCACCATGACAGTTTCAACCCACAACAAAACTTATGCACAGA61541471.4781878358264844No Hit
GAAGAGTTTTTTCCACCAAGATCCCAGAAGACAATCTCTTTGTTATCTAT52048311.2501680365666592No Hit
AGATACAAAGAACCTTTTGTAGTCCAACCAAATTAGGCAGGGAAGAATGG45171051.0849805284389522No Hit
TCCAAACAGGAGTTTTGAAGAAGTCTTAGAGGAGTTCAGAACTTCAGTAG44246821.0627810986316055No Hit
CTGCCTCATATCCATATCAATACCACCACTTCACAGACACACACACACAC44065751.0584319098417847No Hit
TAATTTACTCTCCCCCGTAAGGACCATCAGAAAGAAAAGACTAATGCCTC43609311.0474685049087429No Hit
GAGAAAGAGGGAGTCATCCAGGTATGTGCCTTGGAAGTTTGAGCCACTTG41691401.0014014994860587No Hit
AGACAACCCCATTTTATGAGTTGCTCAGGACCTAAACTCCATCATCACAC40363940.9695167358535645No Hit
GTAGTGGGGAAATAAAGGAAAATAAGAATGGGAAGAAAGTCCTAATACAT31624770.7596073074759236No Hit
GTGTTGCACACACCAAATATTTATAGCCTATCATGTACCAGAGTGATGGA29447670.707314719447431No Hit
CTCTCTCTCTCTCTCACATCCATCCTATTGTTTTTCAATGTAATTCACAA5794120.1391711589488998No Hit
GCTCAAAAATGGTTGTGTCGTTGATGCCGCCTGCGTGCTGCAGTACCCCT5783420.1389141516033921No Hit
TGTATCACAAATGAATGCTGCTGAAATCATAGGATTGATTTTGGGGCCTT5287440.127001020460876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)