FastQCFastQC Report
Fri 10 May 2024
EGAF00002530494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002530494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416330513
Total Bases59.1 Gbp
Sequences flagged as poor quality0
Sequence length1-144
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATCACAAATGAATGCTGCTGGTTTATAAAAGAGAGAATGACCAAGTC99873642.3989027198686252No Hit
ATATTCCAGAAGCAGTGCTCAAGATTTTGAACCAATTAGAGATTTGGGCT96009312.3060839165540576No Hit
GACTTTTTTGGGTCCTAACAGAGAACATTTGGTATGAGAATGTTTCTTGA95259682.288078270160323No Hit
AATTGACTCACTTCTGAACGTTAGGGTCTTCAAAATGATATGTATATACT90655692.177493293651527No Hit
AGGAAATGGTAATATCTCAGGTTTTCCTTATAAAGTTGACATTACCTAGG89604092.152234515657516No Hit
AACACAGAGTTTGTCACACTGAGAAAAATAAAAAACAAGAGACAAGCATG82334311.9776189212439492No Hit
ATTTATTCTTCTCTCCTGGCCCCCTGCTTCCTCATTAGCATTCCTAAGTG81000521.9455821149481782No Hit
AGATTTCAAAGAACTTGGGTCATTTCCCCATCCATGCCAATTCTTGGGTT77682751.8658913429196577No Hit
ATAAAGTCTGGTTGGAAGACAGCCATGCTTATTTGGCTGCTTTCACAGCG74895781.7989500567785672No Hit
AACCAGAGGAAGTAGAAAGAATGAAATAATAAAAATAAAAACAAAATATA73676771.7696701946993731No Hit
ATGAAAAGTCTGTGTAGGCAAATCGGAAGGCCAAAACAAAGGAGCAAAAT72721171.7467172770015058No Hit
ATCTCCAAAATTTGTGAATACATGACCTTATATCACAAAAGATACTTTGC70742411.6991886924223591No Hit
ACTACCACCACTGTCAAGATATAGATCATTTCTGCCACTCCAGAGAATAC70464581.6925153886090498No Hit
AAGTTTCAGGTGATTTCTGAGAATGCTTTTTCTCTTCTGAGTGTACCAAA68860181.6539786984097415No Hit
TTCTCATCACACTTGCTCATCTGTTCATGTGGTGCACACCTGTACAAATT67969681.6325894422251968No Hit
AACGGGGTGAACATCTAGAAAAAAAGAGCATGTGACCTATAAGGAAAAGA67837171.6294066344351752No Hit
TGCTTACTGAACTGTGTTCTTTGTAGAGACAGTTTGTATCTGGGTATAAA63691071.5298198909576441No Hit
AGATACTATCACCATGACAGTTTCAACCCACAACAAAACTTATGCACAGA61541481.4781880760202653No Hit
GAAGAGTTTTTTCCACCAAGATCCCAGAAGACAATCTCTTTGTTATCTAT52048331.250168516954221No Hit
AGATACAAAGAACCTTTTGTAGTCCAACCAAATTAGGCAGGGAAGAATGG45171061.0849807686327329No Hit
TCCAAACAGGAGTTTTGAAGAAGTCTTAGAGGAGTTCAGAACTTCAGTAG44246831.0627813388253864No Hit
CTGCCTCATATCCATATCAATACCACCACTTCACAGACACACACACACAC44065791.0584328706169082No Hit
TAATTTACTCTCCCCCGTAAGGACCATCAGAAAGAAAAGACTAATGCCTC43609321.0474687451025237No Hit
GAGAAAGAGGGAGTCATCCAGGTATGTGCCTTGGAAGTTTGAGCCACTTG41691441.001402460261182No Hit
AGACAACCCCATTTTATGAGTTGCTCAGGACCTAAACTCCATCATCACAC40363950.9695169760473453No Hit
GTAGTGGGGAAATAAAGGAAAATAAGAATGGGAAGAAAGTCCTAATACAT31624790.7596077878634853No Hit
GTGTTGCACACACCAAATATTTATAGCCTATCATGTACCAGAGTGATGGA29447690.7073151998349927No Hit
CTCTCTCTCTCTCTCACATCCATCCTATTGTTTTTCAATGTAATTCACAA5794120.1391711589488998No Hit
GCTCAAAAATGGTTGTGTCGTTGATGCCGCCTGCGTGCTGCAGTACCCCT5783430.13891439179717294No Hit
TGTATCACAAATGAATGCTGCTGAAATCATAGGATTGATTTTGGGGCCTT5287440.127001020460876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)