FastQCFastQC Report
Fri 23 Dec 2022
EGAF00002530497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002530497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373385997
Sequences flagged as poor quality0
Sequence length1-144
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAATGCACCTGATCTGTTCTTAGGCCTCCATCTACATCTGTTACCAG116040463.107788211993392No Hit
AGGTTTTTGATGAGTAAGCCTGCTAGCAGCATACAAACAAACAAGCAAGT107930272.8905816197493874No Hit
CTTCAAGTTGGGTAATGTTGAGCTCATTCACCTCCTTTAATATTACTCTC107400562.876394960253424No Hit
CACCTTGAGGTCCTAAGTCTGTGATTGAAAAAGTACATAGTAGCAAAAAT103223172.764516367227344No Hit
AGATATGGCAGCATCAGATACAAAAAATAAAAGCCATTGTTATTACAAGA88728482.3763205024531224No Hit
CCAGCTCTTCTTCAAACAATGATGTCAGTTCTTAGAGCACTTTAATATAG87493592.343247757092508No Hit
TGAACAAATTTCAGTAACCGCAGGGGGGCCTGGAACCAATTTCCCGTGGA74931992.0068237856279327No Hit
CTCCCTAAAAGCTCCTGTACAATGCTATTCCCGGGCTCAGTAACATATGC74409031.9928179042022296No Hit
AGGCTATGACAGACCACTTTGTCACCATCTCTAGCAGTGATGCATAGCTG74214161.9875989082686463No Hit
CCCACAGAGGAGCATATACTGAAAAGAAGGCAGTATCACAGAATTTATAC68547321.8358299601685384No Hit
AGGATAGATATGGGGATTGAGAGAGAGGGAGGGACAAAAGATGGCACACA59448951.5921579940771053No Hit
TCCACCACCTGTCAATAGTTTCTTTCATTTCCCATCACTCCCCTGAGGCC56500441.513191186974267No Hit
TAAGCATAACAGCTGAGGTTTATTGGTAAATAAGCATAAGAGCTGAGGTT56132111.50332659636403No Hit
ATGGCCAACTTATTTTTCACAAAGGTGCCGAGAACATACAATAGTGAAAG51061901.3675365549394183No Hit
AAATGCTGCTCCTCACATTCTTTGCTGGGCTCAGGTCTCAGACTTACTGA50484011.3520595417508385No Hit
CATGGTAAAGATGGCAATAGAAACCAACTGTGTCACCCAGAGGAAGCTAC46720351.2512614392446002No Hit
CATGAAGTCAGATGAAGTGCTCTGTCTATAACCGAGGCTGAATCTGCATT44635561.1954267261929483No Hit
CTGACCACAGAGCTATGTGTATCTCAGAAGTGCCCAAGAAACAGCGAGGG38846321.0403796690854477No Hit
CTGACCACAGAGCTATGTGTATCTCAGAAGTGCCCAAGAAACAGCGAGCG38511131.0314026318453502No Hit
GACTTTGCGGAGGTATTAACAGAGGAAAACATCTCCACCTTGGAAACTCT38438641.0294612092804327No Hit
AAGAACAATCAGTTAGTATGGGGGAGCTAAGAACCCAAGGGAGATCCCAA37916201.0154692544616235No Hit
CATGAGAGTAGAGACACATATCTCTTATTTAATGTGGTATTTCTAGTTCC34889210.9344006009952216No Hit
GCTTGGAAAGTCTTCACAGTTGGCTTTGGGCCTGTGCCTTCCAATAGCCA31773130.8509459448207426No Hit
ACAACATTAGAAACAAACAACAGATTGAGACAATTAACGGCTTCATAGGT31221940.836184009332305No Hit
GTGTCCTATGCACCATAAGATGTTAAGAAGCATCCTCAGCCTTTATCCAC30693260.8220249352307661No Hit
ATTATACATTGCAAAGAACCGCTTTCCTTAAATTGTAGATTTTTGGATAT29598420.7927030000538559No Hit
CATGAGATTTCTAGGGTTTTCCACTTGGAATAGGATATACCTTTCAGGCT20295850.5435621625628344No Hit
GTATCTCGCCAATGGATTTGTTTCCTTAGGAGCTGGAAGGCATCCAGTGA10727020.2872903667032805No Hit
GAAAGAGTGTTTCCCTTCATTGAGCCACAGTTTTCTCACCTATAAAATAA9039900.2421060262739312No Hit
TAAGCATAACAGCTGAGGTTTGAACTAGTCCAAGCCCATATTAAACTACT5737430.15365948498598891No Hit
CATGACATACATCACTAATTGATAATAGCATTCTTTTCTGTTTTGCCTCA3799620.10176118093684161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)