FastQCFastQC Report
Fri 30 Dec 2022
EGAF00002530498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002530498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373385997
Sequences flagged as poor quality0
Sequence length1-144
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAATGCACCTGATCTGTTCTTAGGCCTCCATCTACATCTGTTACCAG117428843.144971716762051No Hit
AGGTTTTTGATGAGTAAGCCTGCTAGCAGCATACAAACAAACAAGCAAGT105946062.8374406338543006No Hit
CACCTTGAGGTCCTAAGTCTGTGATTGAAAAAGTACATAGTAGCAAAAAT105307912.8203497411821794No Hit
CTTCAAGTTGGGTAATGTTGAGCTCATTCACCTCCTTTAATATTACTCTC103367422.7683796615436544No Hit
CCAGCTCTTCTTCAAACAATGATGTCAGTTCTTAGAGCACTTTAATATAG86626052.3200133560445226No Hit
AGATATGGCAGCATCAGATACAAAAAATAAAAGCCATTGTTATTACAAGA83545182.237501691848396No Hit
CTCCCTAAAAGCTCCTGTACAATGCTATTCCCGGGCTCAGTAACATATGC78479632.1018364542471044No Hit
TGAACAAATTTCAGTAACCGCAGGGGGGCCTGGAACCAATTTCCCGTGGA78092832.0914772012727623No Hit
AGGCTATGACAGACCACTTTGTCACCATCTCTAGCAGTGATGCATAGCTG75415492.0197728518458606No Hit
CCCACAGAGGAGCATATACTGAAAAGAAGGCAGTATCACAGAATTTATAC71859281.924530662032299No Hit
AGGATAGATATGGGGATTGAGAGAGAGGGAGGGACAAAAGATGGCACACA58808541.5750065742288668No Hit
TCCACCACCTGTCAATAGTTTCTTTCATTTCCCATCACTCCCCTGAGGCC57999721.553344808482467No Hit
TAAGCATAACAGCTGAGGTTTATTGGTAAATAAGCATAAGAGCTGAGGTT57855231.5494750865014362No Hit
AAATGCTGCTCCTCACATTCTTTGCTGGGCTCAGGTCTCAGACTTACTGA52549471.407376559973137No Hit
CATGGTAAAGATGGCAATAGAAACCAACTGTGTCACCCAGAGGAAGCTAC45858931.2281909436469842No Hit
ATGGCCAACTTATTTTTCACAAAGGTGCCGAGAACATACAATAGTGAAAG42143401.128681855736545No Hit
GACTTTGCGGAGGTATTAACAGAGGAAAACATCTCCACCTTGGAAACTCT40731911.0908794204191863No Hit
CATGAAGTCAGATGAAGTGCTCTGTCTATAACCGAGGCTGAATCTGCATT40661321.088988883533305No Hit
AAGAACAATCAGTTAGTATGGGGGAGCTAAGAACCCAAGGGAGATCCCAA39733771.0641472984858615No Hit
CTGACCACAGAGCTATGTGTATCTCAGAAGTGCCCAAGAAACAGCGAGGG38282591.0252818881153702No Hit
CTGACCACAGAGCTATGTGTATCTCAGAAGTGCCCAAGAAACAGCGAGCG38204541.0231915579844308No Hit
GCTTGGAAAGTCTTCACAGTTGGCTTTGGGCCTGTGCCTTCCAATAGCCA34801750.9320582528433705No Hit
CATGAGAGTAGAGACACATATCTCTTATTTAATGTGGTATTTCTAGTTCC34291450.9183914307316672No Hit
ACAACATTAGAAACAAACAACAGATTGAGACAATTAACGGCTTCATAGGT31012950.8305868524576727No Hit
GAAAGAGTGTTTCCCTTCATTGAGCCACAGTTTTCTCACCTATAAAATAA28134860.7535060293115384No Hit
GTGTCCTATGCACCATAAGATGTTAAGAAGCATCCTCAGCCTTTATCCAC25477700.6823421393598754No Hit
ATTATACATTGCAAAGAACCGCTTTCCTTAAATTGTAGATTTTTGGATAT25416720.6807089768821727No Hit
CATGAGATTTCTAGGGTTTTCCACTTGGAATAGGATATACCTTTCAGGCT18739980.5018929512774417No Hit
TAAGCATAACAGCTGAGGTTTGAACTAGTCCAAGCCCATATTAAACTACT5894930.15787763995873685No Hit
GTATCTCGCCAATGGATTTGTTTCCTTAGGAGCTGGAAGGCATCCAGTGA4951310.13260566919439135No Hit
CATGACATACATCACTAATTGATAATAGCATTCTTTTCTGTTTTGCCTCA3933730.10535290641871607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)