FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002530499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002530499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11687767
Sequences flagged as poor quality0
Sequence length1-144
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACAGAGGAGCATATACTGAAAAGAAGGCAGTATCACAGAATTTATAC2660472.276285966344127No Hit
AGGATAGATATGGGGATTGAGAGAGAGGGAGGGACAAAAGATGGCACACA2517972.1543636179605565No Hit
CTGTAATGCACCTGATCTGTTCTTAGGCCTCCATCTACATCTGTTACCAG2517762.154183942920833No Hit
CCAGCTCTTCTTCAAACAATGATGTCAGTTCTTAGAGCACTTTAATATAG2507302.1452344147517657No Hit
CTCCCTAAAAGCTCCTGTACAATGCTATTCCCGGGCTCAGTAACATATGC2443702.0908185455784665No Hit
CTTCAAGTTGGGTAATGTTGAGCTCATTCACCTCCTTTAATATTACTCTC2410792.0626609000675664No Hit
AGGTTTTTGATGAGTAAGCCTGCTAGCAGCATACAAACAAACAAGCAAGT2208781.8898220678081623No Hit
AGATATGGCAGCATCAGATACAAAAAATAAAAGCCATTGTTATTACAAGA2082591.78185448084309No Hit
CACCTTGAGGTCCTAAGTCTGTGATTGAAAAAGTACATAGTAGCAAAAAT2044011.7488456092596645No Hit
AAATGCTGCTCCTCACATTCTTTGCTGGGCTCAGGTCTCAGACTTACTGA2031021.7377314246596463No Hit
AAGAACAATCAGTTAGTATGGGGGAGCTAAGAACCCAAGGGAGATCCCAA1928661.650152676726016No Hit
TGAACAAATTTCAGTAACCGCAGGGGGGCCTGGAACCAATTTCCCGTGGA1894081.620566186851603No Hit
AGGCTATGACAGACCACTTTGTCACCATCTCTAGCAGTGATGCATAGCTG1822411.5592456625803714No Hit
CATGAGAGTAGAGACACATATCTCTTATTTAATGTGGTATTTCTAGTTCC1795381.5361189181817194No Hit
ACAACATTAGAAACAAACAACAGATTGAGACAATTAACGGCTTCATAGGT1770691.5149942670828398No Hit
ATTATACATTGCAAAGAACCGCTTTCCTTAAATTGTAGATTTTTGGATAT1762951.5083719584759003No Hit
CATGAAGTCAGATGAAGTGCTCTGTCTATAACCGAGGCTGAATCTGCATT1726831.4774678516435176No Hit
ATGGCCAACTTATTTTTCACAAAGGTGCCGAGAACATACAATAGTGAAAG1617521.3839427154904782No Hit
CATGGTAAAGATGGCAATAGAAACCAACTGTGTCACCCAGAGGAAGCTAC1492251.276762276318479No Hit
GACTTTGCGGAGGTATTAACAGAGGAAAACATCTCCACCTTGGAAACTCT1438691.2309365852348013No Hit
TCCACCACCTGTCAATAGTTTCTTTCATTTCCCATCACTCCCCTGAGGCC1254081.0729851134096018No Hit
TAAGCATAACAGCTGAGGTTTATTGGTAAATAAGCATAAGAGCTGAGGTT1219391.0433045080381906No Hit
GTGTCCTATGCACCATAAGATGTTAAGAAGCATCCTCAGCCTTTATCCAC1157870.9906682773535784No Hit
CTGACCACAGAGCTATGTGTATCTCAGAAGTGCCCAAGAAACAGCGAGGG1114840.9538520061188762No Hit
CTGACCACAGAGCTATGTGTATCTCAGAAGTGCCCAAGAAACAGCGAGCG1110230.9499077111992392No Hit
GCTTGGAAAGTCTTCACAGTTGGCTTTGGGCCTGTGCCTTCCAATAGCCA1041520.891119749392677No Hit
CATGAGATTTCTAGGGTTTTCCACTTGGAATAGGATATACCTTTCAGGCT1009200.8634669051838559No Hit
GTATCTCGCCAATGGATTTGTTTCCTTAGGAGCTGGAAGGCATCCAGTGA882550.7551057443222473No Hit
GCTTGGAAAGTCTTCACAGTTGGCTTTGGGCCTGTGCCTTCAAATAGCCA826710.7073292956644327No Hit
CATGACATACATCACTAATTGATAATAGCATTCTTTTCTGTTTTGCCTCA536210.4587788240473993No Hit
GAAAGAGTGTTTCCCTTCATTGAGCCACAGTTTTCTCACCTATAAAATAA434360.3716364297816683No Hit
TAAGCATAACAGCTGAGGTTTGAACTAGTCCAAGCCCATATTAAACTACT204440.17491792914762933No Hit
AGGATAGATATGGGGATTGAGGGTGAGGAGAGGAACGTGGGGCCAAAATG173270.14824901968014934No Hit
AAAGTTGCATGGTGCTGGAATGGGGCGACTCTGATTCCATTAACATTCTC163680.14004385953279183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)