FastQCFastQC Report
Fri 10 May 2024
EGAF00002530500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002530500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11687767
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length1-144
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACAGAGGAGCATATACTGAAAAGAAGGCAGTATCACAGAATTTATAC2566292.1957059890054276No Hit
AGGATAGATATGGGGATTGAGAGAGAGGGAGGGACAAAAGATGGCACACA2471752.114817997312917No Hit
CTCCCTAAAAGCTCCTGTACAATGCTATTCCCGGGCTCAGTAACATATGC2378722.0352219547155586No Hit
CTGTAATGCACCTGATCTGTTCTTAGGCCTCCATCTACATCTGTTACCAG2340912.0028718916111177No Hit
CCAGCTCTTCTTCAAACAATGATGTCAGTTCTTAGAGCACTTTAATATAG2306721.9736190839533336No Hit
CTTCAAGTTGGGTAATGTTGAGCTCATTCACCTCCTTTAATATTACTCTC2187381.8715123256649453No Hit
AGGTTTTTGATGAGTAAGCCTGCTAGCAGCATACAAACAAACAAGCAAGT2150891.8402916485244787No Hit
CACCTTGAGGTCCTAAGTCTGTGATTGAAAAAGTACATAGTAGCAAAAAT1997021.7086411801330401No Hit
AAATGCTGCTCCTCACATTCTTTGCTGGGCTCAGGTCTCAGACTTACTGA1984571.6979890170637384No Hit
AAGAACAATCAGTTAGTATGGGGGAGCTAAGAACCCAAGGGAGATCCCAA1917531.6406298996206887No Hit
AGATATGGCAGCATCAGATACAAAAAATAAAAGCCATTGTTATTACAAGA1890001.6170753575084102No Hit
CATGAAGTCAGATGAAGTGCTCTGTCTATAACCGAGGCTGAATCTGCATT1754591.5012191807040645No Hit
TGAACAAATTTCAGTAACCGCAGGGGGGCCTGGAACCAATTTCCCGTGGA1752271.499234199312837No Hit
AGGCTATGACAGACCACTTTGTCACCATCTCTAGCAGTGATGCATAGCTG1719061.4708198751737607No Hit
CATGAGAGTAGAGACACATATCTCTTATTTAATGTGGTATTTCTAGTTCC1713091.4657119704730597No Hit
ACAACATTAGAAACAAACAACAGATTGAGACAATTAACGGCTTCATAGGT1665341.4248572888217228No Hit
ATTATACATTGCAAAGAACCGCTTTCCTTAAATTGTAGATTTTTGGATAT1571351.3444398746142012No Hit
GACTTTGCGGAGGTATTAACAGAGGAAAACATCTCCACCTTGGAAACTCT1451711.2420764376976372No Hit
ATGGCCAACTTATTTTTCACAAAGGTGCCGAGAACATACAATAGTGAAAG1448411.2392529727877017No Hit
CATGGTAAAGATGGCAATAGAAACCAACTGTGTCACCCAGAGGAAGCTAC1413011.2089648946629412No Hit
TCCACCACCTGTCAATAGTTTCTTTCATTTCCCATCACTCCCCTGAGGCC1271391.0877954702553534No Hit
TAAGCATAACAGCTGAGGTTTATTGGTAAATAAGCATAAGAGCTGAGGTT1146600.9810257168884355No Hit
CTGACCACAGAGCTATGTGTATCTCAGAAGTGCCCAAGAAACAGCGAGCG1031230.8823156724462423No Hit
GTGTCCTATGCACCATAAGATGTTAAGAAGCATCCTCAGCCTTTATCCAC1025550.8774558904194445No Hit
CTGACCACAGAGCTATGTGTATCTCAGAAGTGCCCAAGAAACAGCGAGGG1023670.8758473710162087No Hit
GCTTGGAAAGTCTTCACAGTTGGCTTTGGGCCTGTGCCTTCCAATAGCCA996710.8527805182974644No Hit
GAAAGAGTGTTTCCCTTCATTGAGCCACAGTTTTCTCACCTATAAAATAA926300.7925380442645716No Hit
CATGAGATTTCTAGGGTTTTCCACTTGGAATAGGATATACCTTTCAGGCT923860.7904503914220741No Hit
GCTTGGAAAGTCTTCACAGTTGGCTTTGGGCCTGTGCCTTCAAATAGCCA787750.6739952978186509No Hit
GTATCTCGCCAATGGATTTGTTTCCTTAGGAGCTGGAAGGCATCCAGTGA669100.5724789003750673No Hit
CATGACATACATCACTAATTGATAATAGCATTCTTTTCTGTTTTGCCTCA530620.45399604560905427No Hit
TAAGCATAACAGCTGAGGTTTGAACTAGTCCAAGCCCATATTAAACTACT191510.16385508027324638No Hit
AAAGTTGCATGGTGCTGGAATGGGGCGACTCTGATTCCATTAACATTCTC169350.14489508560531708No Hit
AGGATAGATATGGGGATTGAGGGTGAGGAGAGGAACGTGGGGCCAAAATG161420.1381102138671998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)