FastQCFastQC Report
Fri 20 Jan 2023
EGAF00002530503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002530503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344046680
Sequences flagged as poor quality0
Sequence length1-144
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAATGCACCTGATCTGTTCTTAGGCCTCCATCTACATCTGTTACCAG103272683.0017054662466154No Hit
CACCTTGAGGTCCTAAGTCTGTGATTGAAAAAGTACATAGTAGCAAAAAT100207752.9126207525095142No Hit
CTTCAAGTTGGGTAATGTTGAGCTCATTCACCTCCTTTAATATTACTCTC89457692.6001614083298232No Hit
AGGTTTTTGATGAGTAAGCCTGCTAGCAGCATACAAACAAACAAGCAAGT87591512.5459193502463098No Hit
AGGCTATGACAGACCACTTTGTCACCATCTCTAGCAGTGATGCATAGCTG83269882.4203076163967054No Hit
TGAACAAATTTCAGTAACCGCAGGGGGGCCTGGAACCAATTTCCCGTGGA82468812.397023857344009No Hit
AGATATGGCAGCATCAGATACAAAAAATAAAAGCCATTGTTATTACAAGA78085532.269620215489363No Hit
CCAGCTCTTCTTCAAACAATGATGTCAGTTCTTAGAGCACTTTAATATAG61930531.8000618404456048No Hit
CTCCCTAAAAGCTCCTGTACAATGCTATTCCCGGGCTCAGTAACATATGC60057011.7456064392192363No Hit
CCCACAGAGGAGCATATACTGAAAAGAAGGCAGTATCACAGAATTTATAC56640591.646305379258419No Hit
TAAGCATAACAGCTGAGGTTTATTGGTAAATAAGCATAAGAGCTGAGGTT53056531.5421317246834063No Hit
AGGATAGATATGGGGATTGAGAGAGAGGGAGGGACAAAAGATGGCACACA51925521.509257987898619No Hit
CATGGTAAAGATGGCAATAGAAACCAACTGTGTCACCCAGAGGAAGCTAC51096781.4851699775158418No Hit
AAATGCTGCTCCTCACATTCTTTGCTGGGCTCAGGTCTCAGACTTACTGA45362691.3185039309200717No Hit
TCCACCACCTGTCAATAGTTTCTTTCATTTCCCATCACTCCCCTGAGGCC41483471.2057512079465496No Hit
CATGAAGTCAGATGAAGTGCTCTGTCTATAACCGAGGCTGAATCTGCATT39403791.145303596593346No Hit
AAGAACAATCAGTTAGTATGGGGGAGCTAAGAACCCAAGGGAGATCCCAA32412380.9420925090746407No Hit
GACTTTGCGGAGGTATTAACAGAGGAAAACATCTCCACCTTGGAAACTCT32084820.932571708001949No Hit
ATGGCCAACTTATTTTTCACAAAGGTGCCGAGAACATACAATAGTGAAAG30761230.8941004749704313No Hit
CATGAGAGTAGAGACACATATCTCTTATTTAATGTGGTATTTCTAGTTCC30022620.8726321672396317No Hit
GCTTGGAAAGTCTTCACAGTTGGCTTTGGGCCTGTGCCTTCCAATAGCCA29560270.8591935838474011No Hit
CTGACCACAGAGCTATGTGTATCTCAGAAGTGCCCAAGAAACAGCGAGGG28614350.8316996402929975No Hit
CTGACCACAGAGCTATGTGTATCTCAGAAGTGCCCAAGAAACAGCGAGCG28293120.8223628258816507No Hit
ACAACATTAGAAACAAACAACAGATTGAGACAATTAACGGCTTCATAGGT26891360.7816195174445514No Hit
CATGAGATTTCTAGGGTTTTCCACTTGGAATAGGATATACCTTTCAGGCT25983580.7552341443899415No Hit
GTGTCCTATGCACCATAAGATGTTAAGAAGCATCCTCAGCCTTTATCCAC22488380.6536432788713439No Hit
GAAAGAGTGTTTCCCTTCATTGAGCCACAGTTTTCTCACCTATAAAATAA20581210.5982098126916964No Hit
ATTATACATTGCAAAGAACCGCTTTCCTTAAATTGTAGATTTTTGGATAT19094180.554988061503747No Hit
TAAGCATAACAGCTGAGGTTTGAACTAGTCCAAGCCCATATTAAACTACT8780360.2552083920705179No Hit
CATGACATACATCACTAATTGATAATAGCATTCTTTTCTGTTTTGCCTCA5161930.15003574514946633No Hit
AAAGTTGCATGGTGCTGGAATGGGGCGACTCTGATTCCATTAACATTCTC3482250.10121446310715743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)