FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002530782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002530782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48873258
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC1484850.3038164552074675TruSeq Adapter, Index 3 (100% over 51bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT554380.11343217593555968Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)