FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002538402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8745825
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATGAC270301630.9063581766157No Hit
ACTTCATA230079026.307295195135964No Hit
TGCCGTGG219925825.14637555633688No Hit
CTAGACCT139608415.962862280002172No Hit
CTAGCCCT297960.34068827126085877No Hit
GAGATGCC159110.1819268050755646Illumina PCR Primer Index 3 (100% over 8bp)
TGCCGTGA111740.1277638187363685No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)