FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002538427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8991587
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATGAC277548430.867565425324806No Hit
ACTTCATA236246826.27420498739544No Hit
TGCCGTGG225888325.122183659013697No Hit
CTAGACCT143045915.908860137815495No Hit
CTAGCCCT354310.39404612333729294No Hit
GAGATGCC196660.21871556155770946Illumina PCR Primer Index 3 (100% over 8bp)
TGCCGTGA113440.12616237823200732No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)