FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002538429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8991587
Sequences flagged as poor quality0
Sequence length58
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCT1654531.840086738859336Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGC1210881.3466810697599878Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTC270820.30119265931586936Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG206990.23020407854586739No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTT198140.22036154463055296Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTT137830.15328773441217886Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCT102270.1137396546349382Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACACAGGATCCGGAGCTGGTGCTGATAA96140.10692217069133625Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)