FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002538501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8084561
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCATCA242243229.963680155298476No Hit
AGTTCGGC191355123.669201085872196No Hit
TCCGGAAG186736623.097927024114234No Hit
GTAATCTT152614618.877289688333107No Hit
CAGCATCC440600.5449893939819367No Hit
AGTTCGGG337570.41754895534834857No Hit
TCCGGATG172600.21349334861843458No Hit
GCCGGAAG136380.16869190547266572No Hit
GGAATCTT131140.16221041563048383No Hit
CCGCATCA105030.12991428971839042No Hit
CAGCAGCA98190.12145371900836668ABI Solid3 Adapter A (100% over 8bp)
CAGCCTCA82840.10246691193250938No Hit
CAGCATCT81840.10122998639010826RNA PCR Primer, Index 43 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)