FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002538521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8743935
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCATCA266599330.48962509442259No Hit
TCCGGAAG208181123.808628495065438No Hit
AGTTCGGC190153221.746867971914245No Hit
GTAATCTT169560519.39178413380246No Hit
CAGCATCC389820.4458175867043842No Hit
AGTTCGGG380460.43511302405610286No Hit
CCGCATCA207460.23726159903979158No Hit
TCCGGATG195210.22325188830886777No Hit
CAGCATCT167710.1918015172802634RNA PCR Primer, Index 43 (100% over 8bp)
TGTTCGGC118390.1353967064027809No Hit
TCCGGTAG106460.12175296362564453No Hit
GTAATCGT90480.1034774389333864No Hit
GGAATCTT88350.10104146474098903No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)