FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002538526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8300375
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCATCA247569029.82624279023538No Hit
AGTTCGGC194822423.47151785310905No Hit
TCCGGAAG191687823.093872264807313No Hit
GTAATCTT156206018.81914973721067No Hit
CAGCATCC495640.5971296477568785No Hit
AGTTCGGG375080.451883198048281No Hit
TCCGGATG184110.22180925560592143No Hit
GCCGGAAG160160.19295513756908575No Hit
GGAATCTT143790.17323313705706067No Hit
CCGCATCA119780.14430673312952727No Hit
CAGCAGCA105610.12723521527641823ABI Solid3 Adapter A (100% over 8bp)
CAGCCTCA93960.11319970483261298No Hit
CAGCATCT93760.11295875186362063RNA PCR Primer, Index 43 (100% over 8bp)
GTAATGTT93460.11259732241013208No Hit
GTAATCGT83480.10057376925741307No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)