FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002538532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6868874
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCATCA212274430.903813346991075No Hit
TCCGGAAG165287324.063230742040105No Hit
AGTTCGGC138241220.125744044802683No Hit
GTAATCTT135982819.796956531740136No Hit
CAGCATCC425630.6196503240560244No Hit
AGTTCGGG277790.4044185407972253No Hit
TCCGGATG173440.25250135611746555No Hit
GCCGGAAG122900.17892306657539503No Hit
GGAATCTT118260.17216795649476174No Hit
CCGCATCA117780.17146915200366175No Hit
CAGCCTCA110280.1605503318302243No Hit
CAGCAGCA95850.13954252181653062ABI Solid3 Adapter A (100% over 8bp)
TCCGGACG79800.11617624664537447No Hit
CAGCATCT75200.10947937027233284RNA PCR Primer, Index 43 (100% over 8bp)
CAGCTTCA74010.10774691747148077No Hit
GTAATCGT72860.10607269837822036No Hit
TGTTCGGC70620.10281161075308703No Hit
GTAATGTT70500.10263690963031205No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)