FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002538539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002538539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7247252
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCATCA226581131.26441580891626No Hit
TCCGGAAG180227124.86833630181481No Hit
GTAATCTT146901220.26991748044638No Hit
AGTTCGGC134953918.62138918310002No Hit
CAGCATCC378480.5222393260231603No Hit
AGTTCGGG317570.43819367672050036No Hit
CCGCATCA210410.29033073501514783No Hit
TCCGGATG184850.255062194608384No Hit
CAGCATCT142510.1966400506012486RNA PCR Primer, Index 43 (100% over 8bp)
TGTTCGGC93990.1296905364957642No Hit
GGAATCTT85710.11826551636399563No Hit
GTAATCGT84170.1161405730061546No Hit
TCCGGTAG83850.11569902633439544No Hit
GTAATGTT77710.10722684957001634No Hit
TCCGGCAG75330.1039428461988075No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)